Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.

The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; fol...

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Main Author: Mahmood, Marziah
Format: Article
Language:English
English
Published: 1983
Online Access:http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf
http://psasir.upm.edu.my/id/eprint/2221/
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spelling my.upm.eprints.22212015-04-13T04:27:37Z http://psasir.upm.edu.my/id/eprint/2221/ Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. Mahmood, Marziah The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2 + (49%). The Mn-deficient enzyme was more sensitive to manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme. However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient enzyme achieved a maximum activity of 105% of control at 2.0mM. 1983 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf Mahmood, Marziah (1983) Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. Pertanika, 6 (1). pp. 63-66. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2 + (49%). The Mn-deficient enzyme was more sensitive to manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme. However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient enzyme achieved a maximum activity of 105% of control at 2.0mM.
format Article
author Mahmood, Marziah
spellingShingle Mahmood, Marziah
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
author_facet Mahmood, Marziah
author_sort Mahmood, Marziah
title Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
title_short Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
title_full Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
title_fullStr Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
title_full_unstemmed Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
title_sort properties of glutamate dehydrogenase in developing legume fruit. ii.the effects of divalent cations.
publishDate 1983
url http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf
http://psasir.upm.edu.my/id/eprint/2221/
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score 13.223943