Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; fol...
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1983
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my.upm.eprints.22212015-04-13T04:27:37Z http://psasir.upm.edu.my/id/eprint/2221/ Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. Mahmood, Marziah The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2 + (49%). The Mn-deficient enzyme was more sensitive to manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme. However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient enzyme achieved a maximum activity of 105% of control at 2.0mM. 1983 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf Mahmood, Marziah (1983) Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. Pertanika, 6 (1). pp. 63-66. English |
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The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus
angustifolius could be partially or fully restored by the addition of divalent cations to the incubation
mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed
by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2
+ (49%). The Mn-deficient enzyme was more sensitive to
manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme.
However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient
enzyme achieved a maximum activity of 105% of control at 2.0mM. |
format |
Article |
author |
Mahmood, Marziah |
spellingShingle |
Mahmood, Marziah Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
author_facet |
Mahmood, Marziah |
author_sort |
Mahmood, Marziah |
title |
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
title_short |
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
title_full |
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
title_fullStr |
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
title_full_unstemmed |
Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations. |
title_sort |
properties of glutamate dehydrogenase in developing legume fruit. ii.the effects of divalent cations. |
publishDate |
1983 |
url |
http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf http://psasir.upm.edu.my/id/eprint/2221/ |
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13.223943 |