Properties of glutamate dehydrogenase in developing legume fruit. II.The effects of divalent cations.
The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus angustifolius could be partially or fully restored by the addition of divalent cations to the incubation mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; fol...
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| Format: | Article |
| Language: | English English |
| Published: |
1983
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| Online Access: | http://psasir.upm.edu.my/id/eprint/2221/1/Properties_of_glutamate_dehydrogenase_in_developing_legume_fruit._II.pdf http://psasir.upm.edu.my/id/eprint/2221/ |
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| Summary: | The activity of EDTA-inhibited glutamate dehydrogenase from a crude seed extract of Lupinus
angustifolius could be partially or fully restored by the addition of divalent cations to the incubation
mixture. Mn2+ and Ca2+ gave the highest stimulations of 67% and 66% of the control, respectively; followed
by Zn2+ (59%), Co2+ (55%), Mg2+ (53%) and Cu 2
+ (49%). The Mn-deficient enzyme was more sensitive to
manganese and more stimulated at a lower manganese concentration than the Mn-sufficient enzyme.
However, Mn-deficient enzyme achieved a maximum activity of 91% at 1.4mM Mn2+ while Mn-sufficient
enzyme achieved a maximum activity of 105% of control at 2.0mM. |
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