Cover Image

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.

Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflamm...

Full description

Saved in:
Bibliographic Details
Main Authors: Hyde, Stephen C., Pringle, Ian A., Abdullah, Syahril, Lawton, Anna E., Davies, Lee A., Varathalingam, Anusha, Nunez-Alonso, Graciela A., Green, Anne Marie, Bazzani, Reto P., Sumner-Jones, Stephanie G., Chan, Mario, Hong, Yu Li, Yew, Nelson S., Cheng, Seng H., Boyd, Christopher A., Davies, Jane C., Griesenbach, Uta, Porteous, David John, Sheppard, David N., Munkonge, Felix M., Alton, Eric W. F. W., Gill, Deborah R.
Format: Article
Language:English
English
Published: Nature Publishing Group 2008
Online Access:http://psasir.upm.edu.my/id/eprint/13817/1/CpG.pdf
http://psasir.upm.edu.my/id/eprint/13817/
http://www.nature.com/nbt/index.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.13817
record_format eprints
spelling my.upm.eprints.138172015-09-17T00:37:15Z http://psasir.upm.edu.my/id/eprint/13817/ CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Hyde, Stephen C. Pringle, Ian A. Abdullah, Syahril Lawton, Anna E. Davies, Lee A. Varathalingam, Anusha Nunez-Alonso, Graciela A. Green, Anne Marie Bazzani, Reto P. Sumner-Jones, Stephanie G. Chan, Mario Hong, Yu Li Yew, Nelson S. Cheng, Seng H. Boyd, Christopher A. Davies, Jane C. Griesenbach, Uta Porteous, David John Sheppard, David N. Munkonge, Felix M. Alton, Eric W. F. W. Gill, Deborah R. Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (≥56 d) in vivo transgene expression in the absence of lung inflammation. Nature Publishing Group 2008-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/13817/1/CpG.pdf Hyde, Stephen C. and Pringle, Ian A. and Abdullah, Syahril and Lawton, Anna E. and Davies, Lee A. and Varathalingam, Anusha and Nunez-Alonso, Graciela A. and Green, Anne Marie and Bazzani, Reto P. and Sumner-Jones, Stephanie G. and Chan, Mario and Hong, Yu Li and Yew, Nelson S. and Cheng, Seng H. and Boyd, Christopher A. and Davies, Jane C. and Griesenbach, Uta and Porteous, David John and Sheppard, David N. and Munkonge, Felix M. and Alton, Eric W. F. W. and Gill, Deborah R. (2008) CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nature Biotechnology, 26 (5). pp. 549-551. ISSN 1087-0156; ESSN: 1546-1696 http://www.nature.com/nbt/index.html 10.1038/nbt1399 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (≥56 d) in vivo transgene expression in the absence of lung inflammation.
format Article
author Hyde, Stephen C.
Pringle, Ian A.
Abdullah, Syahril
Lawton, Anna E.
Davies, Lee A.
Varathalingam, Anusha
Nunez-Alonso, Graciela A.
Green, Anne Marie
Bazzani, Reto P.
Sumner-Jones, Stephanie G.
Chan, Mario
Hong, Yu Li
Yew, Nelson S.
Cheng, Seng H.
Boyd, Christopher A.
Davies, Jane C.
Griesenbach, Uta
Porteous, David John
Sheppard, David N.
Munkonge, Felix M.
Alton, Eric W. F. W.
Gill, Deborah R.
spellingShingle Hyde, Stephen C.
Pringle, Ian A.
Abdullah, Syahril
Lawton, Anna E.
Davies, Lee A.
Varathalingam, Anusha
Nunez-Alonso, Graciela A.
Green, Anne Marie
Bazzani, Reto P.
Sumner-Jones, Stephanie G.
Chan, Mario
Hong, Yu Li
Yew, Nelson S.
Cheng, Seng H.
Boyd, Christopher A.
Davies, Jane C.
Griesenbach, Uta
Porteous, David John
Sheppard, David N.
Munkonge, Felix M.
Alton, Eric W. F. W.
Gill, Deborah R.
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
author_facet Hyde, Stephen C.
Pringle, Ian A.
Abdullah, Syahril
Lawton, Anna E.
Davies, Lee A.
Varathalingam, Anusha
Nunez-Alonso, Graciela A.
Green, Anne Marie
Bazzani, Reto P.
Sumner-Jones, Stephanie G.
Chan, Mario
Hong, Yu Li
Yew, Nelson S.
Cheng, Seng H.
Boyd, Christopher A.
Davies, Jane C.
Griesenbach, Uta
Porteous, David John
Sheppard, David N.
Munkonge, Felix M.
Alton, Eric W. F. W.
Gill, Deborah R.
author_sort Hyde, Stephen C.
title CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
title_short CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
title_full CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
title_fullStr CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
title_full_unstemmed CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
title_sort cpg-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
publisher Nature Publishing Group
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/13817/1/CpG.pdf
http://psasir.upm.edu.my/id/eprint/13817/
http://www.nature.com/nbt/index.html
_version_ 1643825444426874880
score 13.211869

Similar Items