Optimisation of vitamin B12 extraction from green edible seaweed, Ulva lactuca using Central Composite Design
Vitamin B12, which is essential for brain function and a variety of chemical reactions in the body, can be found naturally only in animal foods. Insufficient intake of vitamin B12 lead to vitamin B12 deficiency which is common among strict vegetarians due to their limited intake of animal-based food...
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| Format: | Conference or Workshop Item |
| Language: | English English English |
| Published: |
2021
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| Online Access: | http://irep.iium.edu.my/94548/1/Book%20Abstract_ISSTEC_2021_30%20Nov.pdf http://irep.iium.edu.my/94548/2/Deny%20Susanti.pdf http://irep.iium.edu.my/94548/3/General%20Schedule%20of%203rd%20ISSTEC%202021_FINAL.pdf http://irep.iium.edu.my/94548/ |
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| Summary: | Vitamin B12, which is essential for brain function and a variety of chemical reactions in the body, can be found naturally only in animal foods. Insufficient intake of vitamin B12 lead to vitamin B12 deficiency which is common among strict vegetarians due to their limited intake of animal-based foods. However, extensive studies showed that macroalgae species, specifically Ulva lactuca are rich in vitamin B12 content and can also be further exploited for its dietary applications in the future. In this study, the best extraction method of vitamin B12 from dried U. lactuca was being developed and optimised to achieve the maximum yield of vitamin B12. Also, the effect of several extraction parameters namely; solvent:solvent (MeOH:H2O), solute:solvent ratio, and pH on the total vitamin B12 content were analysed individually by 2-Level Factorial and Central Composite Design. Results showed that highest vitamin B12, particularly Cyanocobalamin (CN-Cbl) content can be recovered through the ultrasonic-assisted extraction (UAE) of oven-dried U. lactuca, at 3 g: 60 mL solute: solvent, 25: 75 % MeOH: H2O ratios and at pH 4. The extraction of CN-Cbl from oven-dried U. lactuca using UAE method has improved the CN-Cbl content as compared with other types of dried U. lactuca using different extraction methods. |
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