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Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology

The optimum protein extraction from fermented and non-fermented Perah seed (Elateriospermumtapos) was investigated using the response surface methodology (RSM). A box-behnken design with three independent variables which are NaOH concentration (6, 8 and 10%), extraction time (10, 20 and 30 minutes)...

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Main Authors: Mazlan, Hamimah Satirah, Muhamad, Ida Idayu, Hassan, Nor Diana, Tan, Nuraimi Azlan Hadi
Format: Article
Language:English
Published: Penerbit UTM 2014
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/54373/1/IdaIdayuMuhamad2014_OptimizationOfProteinExtractionFromFermented.pdf
http://eprints.utm.my/id/eprint/54373/
http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/3027
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spelling my.utm.543732018-08-12T03:49:26Z http://eprints.utm.my/id/eprint/54373/ Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology Mazlan, Hamimah Satirah Muhamad, Ida Idayu Hassan, Nor Diana Tan, Nuraimi Azlan Hadi TP Chemical technology The optimum protein extraction from fermented and non-fermented Perah seed (Elateriospermumtapos) was investigated using the response surface methodology (RSM). A box-behnken design with three independent variables which are NaOH concentration (6, 8 and 10%), extraction time (10, 20 and 30 minutes) and solvent/meal ratio (50:1, 100:1 and 150:1, v/w) was used to study the response of protein yield. A second-degree equation for independent and response variables was produced from simulation to obtain the contour plot graphs. The best protein extraction procedure from both fermented and non-fermented Perah seed was obtained at 5.5% of solvent extraction, 40:1 ratio of solvent/meal and at 32 minutes of reaction time. Surface concentration and ratio of solvent/meal were found to influence the protein yield from fermented seed; meanwhile only the solvent concentration influenced protein yield from non-fermented seed. The maximum protein yields for both fermented and non-fermented Perah seeds were 18.0 g/100g and 5.0 g/100g seed meal respectively Penerbit UTM 2014 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/54373/1/IdaIdayuMuhamad2014_OptimizationOfProteinExtractionFromFermented.pdf Mazlan, Hamimah Satirah and Muhamad, Ida Idayu and Hassan, Nor Diana and Tan, Nuraimi Azlan Hadi (2014) Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology. Jurnal Teknologi (Sciences and Engineering), 68 (5). pp. 29-33. ISSN 2180-3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/3027 DOI: 10.11113/jt.v68.3027
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mazlan, Hamimah Satirah
Muhamad, Ida Idayu
Hassan, Nor Diana
Tan, Nuraimi Azlan Hadi
Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology
description The optimum protein extraction from fermented and non-fermented Perah seed (Elateriospermumtapos) was investigated using the response surface methodology (RSM). A box-behnken design with three independent variables which are NaOH concentration (6, 8 and 10%), extraction time (10, 20 and 30 minutes) and solvent/meal ratio (50:1, 100:1 and 150:1, v/w) was used to study the response of protein yield. A second-degree equation for independent and response variables was produced from simulation to obtain the contour plot graphs. The best protein extraction procedure from both fermented and non-fermented Perah seed was obtained at 5.5% of solvent extraction, 40:1 ratio of solvent/meal and at 32 minutes of reaction time. Surface concentration and ratio of solvent/meal were found to influence the protein yield from fermented seed; meanwhile only the solvent concentration influenced protein yield from non-fermented seed. The maximum protein yields for both fermented and non-fermented Perah seeds were 18.0 g/100g and 5.0 g/100g seed meal respectively
format Article
author Mazlan, Hamimah Satirah
Muhamad, Ida Idayu
Hassan, Nor Diana
Tan, Nuraimi Azlan Hadi
author_facet Mazlan, Hamimah Satirah
Muhamad, Ida Idayu
Hassan, Nor Diana
Tan, Nuraimi Azlan Hadi
author_sort Mazlan, Hamimah Satirah
title Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology
title_short Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology
title_full Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology
title_fullStr Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology
title_full_unstemmed Optimization of protein extraction from fermented and non fermented Perah seed by using response surface methodology
title_sort optimization of protein extraction from fermented and non fermented perah seed by using response surface methodology
publisher Penerbit UTM
publishDate 2014
url http://eprints.utm.my/id/eprint/54373/1/IdaIdayuMuhamad2014_OptimizationOfProteinExtractionFromFermented.pdf
http://eprints.utm.my/id/eprint/54373/
http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/3027
_version_ 1643653514703929344
score 13.211869

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