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Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)

Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. T...

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Main Authors: Yuswan, Mohd Hafis, A. Jalil, Nurul Hanani, Mohamad, Haslina, Keso, Shamsidah, Mohamad, Nurhidayatul Asma, Tengku Md. Yusoff, Tengku Shahrul, Ismail, Nor Falahiah, Abdul Manaf, Yanty Noorzianna, Mohd Hashim, Amalia, Mohd Desa, Mohd Nasir, Yusof, Yus Aniza, Mustafa, Shuhaimi
Format: Article
Language:English
Published: Elsevier 2021
Online Access:http://psasir.upm.edu.my/id/eprint/97581/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/97581/
https://www.sciencedirect.com/science/article/pii/S0308814620316241
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spelling my.upm.eprints.975812022-11-25T08:20:40Z http://psasir.upm.edu.my/id/eprint/97581/ Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen) Yuswan, Mohd Hafis A. Jalil, Nurul Hanani Mohamad, Haslina Keso, Shamsidah Mohamad, Nurhidayatul Asma Tengku Md. Yusoff, Tengku Shahrul Ismail, Nor Falahiah Abdul Manaf, Yanty Noorzianna Mohd Hashim, Amalia Mohd Desa, Mohd Nasir Yusof, Yus Aniza Mustafa, Shuhaimi Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. Thus, the aim of this study was to investigate the utility of monitoring hydroxyproline, a signature amino acid for gelatin and collagen, for identifying potentially haram foodstuffs. To determine the hydroxyproline profiles among animal- and plant-based samples, one-way univariate analysis of variance followed by pair-wise comparison was used to establish statistical significance. Multivariate chemometric analysis through principal component analysis revealed a discrete distribution pattern among 59 samples due to hydroxyproline variability. Finally, inter- and intra-laboratory comparisons demonstrated the validity and robustness of hydroxyproline determination according to ISO 17025. Thus, this preliminary identification technique will aid the identification of potentially haram foodstuffs. Elsevier 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97581/1/ABSTRACT.pdf Yuswan, Mohd Hafis and A. Jalil, Nurul Hanani and Mohamad, Haslina and Keso, Shamsidah and Mohamad, Nurhidayatul Asma and Tengku Md. Yusoff, Tengku Shahrul and Ismail, Nor Falahiah and Abdul Manaf, Yanty Noorzianna and Mohd Hashim, Amalia and Mohd Desa, Mohd Nasir and Yusof, Yus Aniza and Mustafa, Shuhaimi (2021) Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen). Food Chemistry, 337. art. no. 127762. pp. 1-7. ISSN 0308-8146; ESSN: 1873-7072 https://www.sciencedirect.com/science/article/pii/S0308814620316241 10.1016/j.foodchem.2020.127762
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. Thus, the aim of this study was to investigate the utility of monitoring hydroxyproline, a signature amino acid for gelatin and collagen, for identifying potentially haram foodstuffs. To determine the hydroxyproline profiles among animal- and plant-based samples, one-way univariate analysis of variance followed by pair-wise comparison was used to establish statistical significance. Multivariate chemometric analysis through principal component analysis revealed a discrete distribution pattern among 59 samples due to hydroxyproline variability. Finally, inter- and intra-laboratory comparisons demonstrated the validity and robustness of hydroxyproline determination according to ISO 17025. Thus, this preliminary identification technique will aid the identification of potentially haram foodstuffs.
format Article
author Yuswan, Mohd Hafis
A. Jalil, Nurul Hanani
Mohamad, Haslina
Keso, Shamsidah
Mohamad, Nurhidayatul Asma
Tengku Md. Yusoff, Tengku Shahrul
Ismail, Nor Falahiah
Abdul Manaf, Yanty Noorzianna
Mohd Hashim, Amalia
Mohd Desa, Mohd Nasir
Yusof, Yus Aniza
Mustafa, Shuhaimi
spellingShingle Yuswan, Mohd Hafis
A. Jalil, Nurul Hanani
Mohamad, Haslina
Keso, Shamsidah
Mohamad, Nurhidayatul Asma
Tengku Md. Yusoff, Tengku Shahrul
Ismail, Nor Falahiah
Abdul Manaf, Yanty Noorzianna
Mohd Hashim, Amalia
Mohd Desa, Mohd Nasir
Yusof, Yus Aniza
Mustafa, Shuhaimi
Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
author_facet Yuswan, Mohd Hafis
A. Jalil, Nurul Hanani
Mohamad, Haslina
Keso, Shamsidah
Mohamad, Nurhidayatul Asma
Tengku Md. Yusoff, Tengku Shahrul
Ismail, Nor Falahiah
Abdul Manaf, Yanty Noorzianna
Mohd Hashim, Amalia
Mohd Desa, Mohd Nasir
Yusof, Yus Aniza
Mustafa, Shuhaimi
author_sort Yuswan, Mohd Hafis
title Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
title_short Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
title_full Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
title_fullStr Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
title_full_unstemmed Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
title_sort hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)
publisher Elsevier
publishDate 2021
url http://psasir.upm.edu.my/id/eprint/97581/1/ABSTRACT.pdf
http://psasir.upm.edu.my/id/eprint/97581/
https://www.sciencedirect.com/science/article/pii/S0308814620316241
_version_ 1751538274413838336
score 13.211869

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