Staff View: Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds

Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds

Aspergillus flavus is a toxigenic fungus well known for the synthesis of aflatoxins that contaminate crops and food products. Antifungal peptides generated by lactic acid bacteria have a high potential for applications as bio-control agent to prolong the shelf life of crops. In this study, antifunga...

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Main Authors:	Muhialdin, Belal J., Algboory, Hussein L., Kadum, Hana, Mohammed, Nameer K., Saari, Nazamid, Hassan, Zaiton, Meor Hussin, Anis Shobirin
Format:	Article
Language:	English
Published:	Elsevier 2020
Online Access:	http://psasir.upm.edu.my/id/eprint/87694/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/87694/ https://www.sciencedirect.com/science/article/pii/S0956713519304876
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spelling	my.upm.eprints.876942022-07-06T04:02:12Z http://psasir.upm.edu.my/id/eprint/87694/ Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds Muhialdin, Belal J. Algboory, Hussein L. Kadum, Hana Mohammed, Nameer K. Saari, Nazamid Hassan, Zaiton Meor Hussin, Anis Shobirin Aspergillus flavus is a toxigenic fungus well known for the synthesis of aflatoxins that contaminate crops and food products. Antifungal peptides generated by lactic acid bacteria have a high potential for applications as bio-control agent to prolong the shelf life of crops. In this study, antifungal activity of peptides generated by Lactobacillus plantarum TE10 was tested against the spoilage fungi Aspergillus flavus MD3. L. plantarum TE10 was inoculated in MRS broth and incubated at 37 °C for 48 h and the antifungal activity was determined using dual agar overlay method. The cell free supernatant was fractionated using size exclusion chromatography, and the peptides were identified using LC-MS/MS. Scanning electron microscope was performed to determine the effects of the active fraction on the morphology of target fungi. The antifungal activity of the active fraction was further confirmed against selected fungi in fresh maize seeds. A total of 37 peptides were identified in fraction 7 that showed the highest antifungal activity. The peptides mixture in fraction 7 caused damage at the tip of the mycelia as observed by scanning electron microscope. Growth of A. flavus was observed after 7 days on the samples treated with distilled water and MRS broth, while slight growth was observed on the sample treated with fraction 7. Fraction 7 reduced the spore formation of A. flavus by 4 folds compared to the control. The results demonstrated promising application of the peptides mixture as bio-control agent to prevent the growth of A. flavus in maize. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87694/1/ABSTRACT.pdf Muhialdin, Belal J. and Algboory, Hussein L. and Kadum, Hana and Mohammed, Nameer K. and Saari, Nazamid and Hassan, Zaiton and Meor Hussin, Anis Shobirin (2020) Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds. Food Control, 109. art. no. 106898. pp. 1-6. ISSN 0956-7135 https://www.sciencedirect.com/science/article/pii/S0956713519304876 10.1016/j.foodcont.2019.106898
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Aspergillus flavus is a toxigenic fungus well known for the synthesis of aflatoxins that contaminate crops and food products. Antifungal peptides generated by lactic acid bacteria have a high potential for applications as bio-control agent to prolong the shelf life of crops. In this study, antifungal activity of peptides generated by Lactobacillus plantarum TE10 was tested against the spoilage fungi Aspergillus flavus MD3. L. plantarum TE10 was inoculated in MRS broth and incubated at 37 °C for 48 h and the antifungal activity was determined using dual agar overlay method. The cell free supernatant was fractionated using size exclusion chromatography, and the peptides were identified using LC-MS/MS. Scanning electron microscope was performed to determine the effects of the active fraction on the morphology of target fungi. The antifungal activity of the active fraction was further confirmed against selected fungi in fresh maize seeds. A total of 37 peptides were identified in fraction 7 that showed the highest antifungal activity. The peptides mixture in fraction 7 caused damage at the tip of the mycelia as observed by scanning electron microscope. Growth of A. flavus was observed after 7 days on the samples treated with distilled water and MRS broth, while slight growth was observed on the sample treated with fraction 7. Fraction 7 reduced the spore formation of A. flavus by 4 folds compared to the control. The results demonstrated promising application of the peptides mixture as bio-control agent to prevent the growth of A. flavus in maize.
format	Article
author	Muhialdin, Belal J. Algboory, Hussein L. Kadum, Hana Mohammed, Nameer K. Saari, Nazamid Hassan, Zaiton Meor Hussin, Anis Shobirin
spellingShingle	Muhialdin, Belal J. Algboory, Hussein L. Kadum, Hana Mohammed, Nameer K. Saari, Nazamid Hassan, Zaiton Meor Hussin, Anis Shobirin Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds
author_facet	Muhialdin, Belal J. Algboory, Hussein L. Kadum, Hana Mohammed, Nameer K. Saari, Nazamid Hassan, Zaiton Meor Hussin, Anis Shobirin
author_sort	Muhialdin, Belal J.
title	Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds
title_short	Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds
title_full	Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds
title_fullStr	Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds
title_full_unstemmed	Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds
title_sort	antifungal activity determination for the peptides generated by lactobacillus plantarum te10 against aspergillus flavus in maize seeds
publisher	Elsevier
publishDate	2020
url	http://psasir.upm.edu.my/id/eprint/87694/1/ABSTRACT.pdf http://psasir.upm.edu.my/id/eprint/87694/ https://www.sciencedirect.com/science/article/pii/S0956713519304876
_version_	1738511967985860608
score	13.211869

Antifungal activity determination for the peptides generated by Lactobacillus plantarum TE10 against Aspergillus flavus in maize seeds

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