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Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)

The ability to detect the presence of transgenes in crop-derived foods depends on the quantity and quality of DNA obtained from a product to be analyzed. The efficiency of DNA extraction protocols differs due to the nature of each food product. In this paper, we described two main DNA extraction pro...

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Main Authors: Tung, Nguyen Chau Thanh, Radu, Son, Abdul Rahim, Raha, Lai, Oi Ming, Wong, Clemente Michael Vui Ling
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2009
Online Access:http://psasir.upm.edu.my/id/eprint/40900/1/03%20IFRJ-2007-145%20Tung%20Vietnam%20FINAL.pdf
http://psasir.upm.edu.my/id/eprint/40900/
http://www.ifrj.upm.edu.my/16%20%281%29%202009/03%20IFRJ-2007-145%20Tung%20Vietnam%20FINAL.pdf
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spelling my.upm.eprints.409002015-10-09T04:45:17Z http://psasir.upm.edu.my/id/eprint/40900/ Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs) Tung, Nguyen Chau Thanh Radu, Son Abdul Rahim, Raha Lai, Oi Ming Wong, Clemente Michael Vui Ling The ability to detect the presence of transgenes in crop-derived foods depends on the quantity and quality of DNA obtained from a product to be analyzed. The efficiency of DNA extraction protocols differs due to the nature of each food product. In this paper, we described two main DNA extraction protocols and their modifications that have been applied and evaluated for DNA extraction from raw and processed food as well as animal feed. The yield and quality for five categories of food and feed samples namely, raw soybean, raw maize, animal feed, smooth tofu and soymilk are discussed. The statistical interaction analyses showed that the cetyltrimethyl ammonium bromide (CTAB) method was proven to be the best method to extract DNA from raw soybean, maize and animal feed samples which not only obtained high DNA yield of 32.7, 28.4 and 33.4 ng DNA/mg sample respectively, but also produced high quality DNA with the absorbance A260/A280 ratio of 1.9, 1.9 and 2.0, respectively. These DNA were suitable for PCR amplification which produced a 164 bp DNA fragment of the lectin gene from soybean, and a 277 bp DNA fragment of the zein gene from maize. In the processed food category, the Wizard isolation method was found to be the best for the extraction of DNA from smooth tofu and soymilk with the yield of 13.2 and 3.4 ng DNA/mg sample, and the quality of the DNA at the absorbance A260/A280 ratio ranged from 1.9 to 1.7. These DNA were successfully amplified using primers specific to the lectin gene of soybean. Faculty of Food Science and Technology, Universiti Putra Malaysia 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40900/1/03%20IFRJ-2007-145%20Tung%20Vietnam%20FINAL.pdf Tung, Nguyen Chau Thanh and Radu, Son and Abdul Rahim, Raha and Lai, Oi Ming and Wong, Clemente Michael Vui Ling (2009) Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs). International Food Research Journal, 16 (1). pp. 21-30. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/16%20%281%29%202009/03%20IFRJ-2007-145%20Tung%20Vietnam%20FINAL.pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The ability to detect the presence of transgenes in crop-derived foods depends on the quantity and quality of DNA obtained from a product to be analyzed. The efficiency of DNA extraction protocols differs due to the nature of each food product. In this paper, we described two main DNA extraction protocols and their modifications that have been applied and evaluated for DNA extraction from raw and processed food as well as animal feed. The yield and quality for five categories of food and feed samples namely, raw soybean, raw maize, animal feed, smooth tofu and soymilk are discussed. The statistical interaction analyses showed that the cetyltrimethyl ammonium bromide (CTAB) method was proven to be the best method to extract DNA from raw soybean, maize and animal feed samples which not only obtained high DNA yield of 32.7, 28.4 and 33.4 ng DNA/mg sample respectively, but also produced high quality DNA with the absorbance A260/A280 ratio of 1.9, 1.9 and 2.0, respectively. These DNA were suitable for PCR amplification which produced a 164 bp DNA fragment of the lectin gene from soybean, and a 277 bp DNA fragment of the zein gene from maize. In the processed food category, the Wizard isolation method was found to be the best for the extraction of DNA from smooth tofu and soymilk with the yield of 13.2 and 3.4 ng DNA/mg sample, and the quality of the DNA at the absorbance A260/A280 ratio ranged from 1.9 to 1.7. These DNA were successfully amplified using primers specific to the lectin gene of soybean.
format Article
author Tung, Nguyen Chau Thanh
Radu, Son
Abdul Rahim, Raha
Lai, Oi Ming
Wong, Clemente Michael Vui Ling
spellingShingle Tung, Nguyen Chau Thanh
Radu, Son
Abdul Rahim, Raha
Lai, Oi Ming
Wong, Clemente Michael Vui Ling
Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)
author_facet Tung, Nguyen Chau Thanh
Radu, Son
Abdul Rahim, Raha
Lai, Oi Ming
Wong, Clemente Michael Vui Ling
author_sort Tung, Nguyen Chau Thanh
title Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)
title_short Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)
title_full Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)
title_fullStr Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)
title_full_unstemmed Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)
title_sort comparison of dna extraction efficiencies using various methods for the detection of genetically modified organisms (gmos)
publisher Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/40900/1/03%20IFRJ-2007-145%20Tung%20Vietnam%20FINAL.pdf
http://psasir.upm.edu.my/id/eprint/40900/
http://www.ifrj.upm.edu.my/16%20%281%29%202009/03%20IFRJ-2007-145%20Tung%20Vietnam%20FINAL.pdf
_version_ 1643832844690128896
score 13.211869

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