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Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application

Recent trends in anti-aging research projected the use of antioxidant compound derived from botanical products. Phenolic acids, flavonoids and high molecular weight polyphenols are some of antioxidants which are able to protect human skin against the harmful effects of ultraviolet irradiation, i.e....

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Main Authors: Abdul Wahab, Norliza, Abdul Rahman, Russly, Ismail, Amin, Mustafa, Shuhaimi, Hashim, Puziah
Format: Article
Language:English
Published: Omics Publishing Group 2014
Online Access:http://psasir.upm.edu.my/id/eprint/36529/1/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.pdf
http://psasir.upm.edu.my/id/eprint/36529/
http://www.esciencecentral.org/journals/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.php?aid=25764
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spelling my.upm.eprints.365292016-04-06T04:39:38Z http://psasir.upm.edu.my/id/eprint/36529/ Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application Abdul Wahab, Norliza Abdul Rahman, Russly Ismail, Amin Mustafa, Shuhaimi Hashim, Puziah Recent trends in anti-aging research projected the use of antioxidant compound derived from botanical products. Phenolic acids, flavonoids and high molecular weight polyphenols are some of antioxidants which are able to protect human skin against the harmful effects of ultraviolet irradiation, i.e. photoaging and skin cancer development. Various studies have demonstrated cocoa beans to contain polyphenols and possess health promoting effects mainly on antiradical property. In this study, the HPLC/DAD quantification of (–)-epicatechin from PBC123 and PBC140 Malaysian unfermented cocoa bean extracts (CBEs) were 121.01 and 118.09 mg/g DW, whereas concentration of (+)-catechin were 6.65 and 6.53 mg/g DW, respectively with no significant differences (p>0.05). In term of antioxidant capacity, Ferric reducing/antioxidant power of the respective clones were assayed at 822.10 and 795.99 mM FeSO4/g DW. Inhibition of proteinases expression induces by reactive oxygen species were exhibited in the anti-collagenase and anti-elastase assays. The anti-collagenase activity of PBC123 and PBC140 were 62.99% and 59.96% whereas anti-elastase has been measured at 36.60% and 15.75%, respectively. Positive and high correlation were observed within epicatechin content (1), FRAP (2) and anti-collagenase (3) with significant relationships for both PBC123 and PBC140 (r12=0.901, r13=0.768 and r23=0.908). A statistical One-Way ANOVA showed that there was no significant difference obtained between PBC123 and PBC140 in terms of epicatechin, FRAP and anti-collagenase assays, however, significant difference was observed from anti-elastase assessment (p<0.05).These results indicate unfermented PBC123 clone as a potential source of natural ingredient in a cosmetic industry. Omics Publishing Group 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36529/1/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.pdf Abdul Wahab, Norliza and Abdul Rahman, Russly and Ismail, Amin and Mustafa, Shuhaimi and Hashim, Puziah (2014) Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application. Natural Products Chemistry & Research, 2 (3). art. no. 1000132. pp. 1-6. ISSN 2329-6836 http://www.esciencecentral.org/journals/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.php?aid=25764 10.4172/2329-6836.1000132
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Recent trends in anti-aging research projected the use of antioxidant compound derived from botanical products. Phenolic acids, flavonoids and high molecular weight polyphenols are some of antioxidants which are able to protect human skin against the harmful effects of ultraviolet irradiation, i.e. photoaging and skin cancer development. Various studies have demonstrated cocoa beans to contain polyphenols and possess health promoting effects mainly on antiradical property. In this study, the HPLC/DAD quantification of (–)-epicatechin from PBC123 and PBC140 Malaysian unfermented cocoa bean extracts (CBEs) were 121.01 and 118.09 mg/g DW, whereas concentration of (+)-catechin were 6.65 and 6.53 mg/g DW, respectively with no significant differences (p>0.05). In term of antioxidant capacity, Ferric reducing/antioxidant power of the respective clones were assayed at 822.10 and 795.99 mM FeSO4/g DW. Inhibition of proteinases expression induces by reactive oxygen species were exhibited in the anti-collagenase and anti-elastase assays. The anti-collagenase activity of PBC123 and PBC140 were 62.99% and 59.96% whereas anti-elastase has been measured at 36.60% and 15.75%, respectively. Positive and high correlation were observed within epicatechin content (1), FRAP (2) and anti-collagenase (3) with significant relationships for both PBC123 and PBC140 (r12=0.901, r13=0.768 and r23=0.908). A statistical One-Way ANOVA showed that there was no significant difference obtained between PBC123 and PBC140 in terms of epicatechin, FRAP and anti-collagenase assays, however, significant difference was observed from anti-elastase assessment (p<0.05).These results indicate unfermented PBC123 clone as a potential source of natural ingredient in a cosmetic industry.
format Article
author Abdul Wahab, Norliza
Abdul Rahman, Russly
Ismail, Amin
Mustafa, Shuhaimi
Hashim, Puziah
spellingShingle Abdul Wahab, Norliza
Abdul Rahman, Russly
Ismail, Amin
Mustafa, Shuhaimi
Hashim, Puziah
Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
author_facet Abdul Wahab, Norliza
Abdul Rahman, Russly
Ismail, Amin
Mustafa, Shuhaimi
Hashim, Puziah
author_sort Abdul Wahab, Norliza
title Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
title_short Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
title_full Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
title_fullStr Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
title_full_unstemmed Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
title_sort assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of malaysian unfermented cocoa bean for cosmetic application
publisher Omics Publishing Group
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/36529/1/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.pdf
http://psasir.upm.edu.my/id/eprint/36529/
http://www.esciencecentral.org/journals/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.php?aid=25764
_version_ 1643831763723616256
score 13.211869

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