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Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1

A locally isolated Acinetobacter sp. Strain AQ5NOL 1 was encapsulated in gellan gum and its ability to degrade phenol was compared with the free cells. Optimal phenol degradation was achieved at gellan gum concentration of 0.75% (w/v), bead size of 3 mm diameter (estimated surface area of 28.26 mm2)...

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Main Authors: Ahmad, Siti Aqlima, Shamaan, Nor Aripin, Mat Arif, Noorliza, Gan, Bee Koon, Abd. Shukor, Mohd. Yunus, Syed, Mohd Arif
Format: Article
Language:English
Published: Springer 2012
Online Access:http://psasir.upm.edu.my/id/eprint/22396/1/Enhanced%20phenol%20degradation%20by%20immobilized%20Acinetobacter%20sp.%20strain%20AQ5NOL%201.pdf
http://psasir.upm.edu.my/id/eprint/22396/
http://link.springer.com/article/10.1007%2Fs11274-011-0826-z
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spelling my.upm.eprints.223962016-09-21T04:49:42Z http://psasir.upm.edu.my/id/eprint/22396/ Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1 Ahmad, Siti Aqlima Shamaan, Nor Aripin Mat Arif, Noorliza Gan, Bee Koon Abd. Shukor, Mohd. Yunus Syed, Mohd Arif A locally isolated Acinetobacter sp. Strain AQ5NOL 1 was encapsulated in gellan gum and its ability to degrade phenol was compared with the free cells. Optimal phenol degradation was achieved at gellan gum concentration of 0.75% (w/v), bead size of 3 mm diameter (estimated surface area of 28.26 mm2) and bead number of 300 per 100 ml medium. At phenol concentration of 100 mg l−1, both free and immobilized bacteria exhibited similar rates of phenol degradation but at higher phenol concentrations, the immobilized bacteria exhibited a higher rate of degradation of phenol. The immobilized cells completely degrade phenol within 108, 216 and 240 h at 1,100, 1,500 and 1,900 mg l−1 phenol, respectively, whereas free cells took 240 h to completely degrade phenol at 1,100 mg l−1. However, the free cells were unable to completely degrade phenol at higher concentrations. Overall, the rates of phenol degradation by both immobilized and free bacteria decreased gradually as the phenol concentration was increased. The immobilized cells showed no loss in phenol degrading activity after being used repeatedly for 45 cycles of 18 h cycle. However, phenol degrading activity of the immobilized bacteria experienced 10 and 38% losses after the 46 and 47th cycles, respectively. The study has shown an increased efficiency of phenol degradation when the cells are encapsulated in gellan gum. Springer 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22396/1/Enhanced%20phenol%20degradation%20by%20immobilized%20Acinetobacter%20sp.%20strain%20AQ5NOL%201.pdf Ahmad, Siti Aqlima and Shamaan, Nor Aripin and Mat Arif, Noorliza and Gan, Bee Koon and Abd. Shukor, Mohd. Yunus and Syed, Mohd Arif (2012) Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1. World Journal of Microbiology and Biotechnology, 28 (1). pp. 347-352. ISSN 0959-3993; ESSN: 1573-0972 http://link.springer.com/article/10.1007%2Fs11274-011-0826-z 10.1007/s11274-011-0826-z
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A locally isolated Acinetobacter sp. Strain AQ5NOL 1 was encapsulated in gellan gum and its ability to degrade phenol was compared with the free cells. Optimal phenol degradation was achieved at gellan gum concentration of 0.75% (w/v), bead size of 3 mm diameter (estimated surface area of 28.26 mm2) and bead number of 300 per 100 ml medium. At phenol concentration of 100 mg l−1, both free and immobilized bacteria exhibited similar rates of phenol degradation but at higher phenol concentrations, the immobilized bacteria exhibited a higher rate of degradation of phenol. The immobilized cells completely degrade phenol within 108, 216 and 240 h at 1,100, 1,500 and 1,900 mg l−1 phenol, respectively, whereas free cells took 240 h to completely degrade phenol at 1,100 mg l−1. However, the free cells were unable to completely degrade phenol at higher concentrations. Overall, the rates of phenol degradation by both immobilized and free bacteria decreased gradually as the phenol concentration was increased. The immobilized cells showed no loss in phenol degrading activity after being used repeatedly for 45 cycles of 18 h cycle. However, phenol degrading activity of the immobilized bacteria experienced 10 and 38% losses after the 46 and 47th cycles, respectively. The study has shown an increased efficiency of phenol degradation when the cells are encapsulated in gellan gum.
format Article
author Ahmad, Siti Aqlima
Shamaan, Nor Aripin
Mat Arif, Noorliza
Gan, Bee Koon
Abd. Shukor, Mohd. Yunus
Syed, Mohd Arif
spellingShingle Ahmad, Siti Aqlima
Shamaan, Nor Aripin
Mat Arif, Noorliza
Gan, Bee Koon
Abd. Shukor, Mohd. Yunus
Syed, Mohd Arif
Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1
author_facet Ahmad, Siti Aqlima
Shamaan, Nor Aripin
Mat Arif, Noorliza
Gan, Bee Koon
Abd. Shukor, Mohd. Yunus
Syed, Mohd Arif
author_sort Ahmad, Siti Aqlima
title Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1
title_short Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1
title_full Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1
title_fullStr Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1
title_full_unstemmed Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1
title_sort enhanced phenol degradation by immobilized acinetobacter sp. strain aq5nol 1
publisher Springer
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/22396/1/Enhanced%20phenol%20degradation%20by%20immobilized%20Acinetobacter%20sp.%20strain%20AQ5NOL%201.pdf
http://psasir.upm.edu.my/id/eprint/22396/
http://link.springer.com/article/10.1007%2Fs11274-011-0826-z
_version_ 1643827815506771968
score 13.211869

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