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Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin

The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The op...

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Main Authors: Lee, Kong H., Lee, Pat M., Siaw, Y.S., Morihara, Kazuyuki
Format: Article
Language:English
Published: Springer Science and Business Media LLC 1992
Online Access:http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf
http://psasir.upm.edu.my/id/eprint/115092/
https://link.springer.com/article/10.1007/BF01027021?error=cookies_not_supported&code=d1721d86-f388-468c-9b3a-08448006333f
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spelling my.upm.eprints.1150922025-02-19T07:44:35Z http://psasir.upm.edu.my/id/eprint/115092/ Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin Lee, Kong H. Lee, Pat M. Siaw, Y.S. Morihara, Kazuyuki The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4°C and 37°C were 50% and 25% respectively where an increase in peptide yield of 85% was obtained for both conditions as compared to that in water. Springer Science and Business Media LLC 1992 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf Lee, Kong H. and Lee, Pat M. and Siaw, Y.S. and Morihara, Kazuyuki (1992) Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin. Biotechnology Letters, 14 (12). pp. 1159-1164. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1007/BF01027021?error=cookies_not_supported&code=d1721d86-f388-468c-9b3a-08448006333f 10.1007/BF01027021
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4°C and 37°C were 50% and 25% respectively where an increase in peptide yield of 85% was obtained for both conditions as compared to that in water.
format Article
author Lee, Kong H.
Lee, Pat M.
Siaw, Y.S.
Morihara, Kazuyuki
spellingShingle Lee, Kong H.
Lee, Pat M.
Siaw, Y.S.
Morihara, Kazuyuki
Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
author_facet Lee, Kong H.
Lee, Pat M.
Siaw, Y.S.
Morihara, Kazuyuki
author_sort Lee, Kong H.
title Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
title_short Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
title_full Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
title_fullStr Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
title_full_unstemmed Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
title_sort aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
publisher Springer Science and Business Media LLC
publishDate 1992
url http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf
http://psasir.upm.edu.my/id/eprint/115092/
https://link.springer.com/article/10.1007/BF01027021?error=cookies_not_supported&code=d1721d86-f388-468c-9b3a-08448006333f
_version_ 1825162432078675968
score 13.239859

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