Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin
The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The op...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC
1992
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| Online Access: | http://psasir.upm.edu.my/id/eprint/115092/1/115092.pdf http://psasir.upm.edu.my/id/eprint/115092/ https://link.springer.com/article/10.1007/BF01027021?error=cookies_not_supported&code=d1721d86-f388-468c-9b3a-08448006333f |
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| Summary: | The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4°C and 37°C were 50% and 25% respectively where an increase in peptide yield of 85% was obtained for both conditions as compared to that in water. |
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