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2D PAGE analysis of chicken samples from different slaughtering process

This study investigated the expression of protein in chicken meat that assumed to be influenced by variations of the method of slaughtering. There were two types of samples that depend on the method of slaughtering had been examined. Samples are: (i) Severing the trachea, oesophagus and carotid arte...

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Main Author: Nur Syafinaz Aqmal, Paijo
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://ir.unimas.my/id/eprint/35039/1/Nur%20Syafinaz%20Aqmal%2024%20pgs.pdf
http://ir.unimas.my/id/eprint/35039/4/Nur%20Syafinaz%20Aqmal%20ft.pdf
http://ir.unimas.my/id/eprint/35039/
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spelling my.unimas.ir.350392024-02-02T07:06:04Z http://ir.unimas.my/id/eprint/35039/ 2D PAGE analysis of chicken samples from different slaughtering process Nur Syafinaz Aqmal, Paijo Q Science (General) QH301 Biology This study investigated the expression of protein in chicken meat that assumed to be influenced by variations of the method of slaughtering. There were two types of samples that depend on the method of slaughtering had been examined. Samples are: (i) Severing the trachea, oesophagus and carotid arteries and jugular veins which are halal method, and (ii) Cut directly the neck of the chicken without considering the veins which are non halal method. Proteins in the sample had been extracted by two different extraction methods and quantified with Bradford assay to know the protein concentration. Protein expressed in higher amounts mostly found distributed at range of Mw 63-100 kDa for direct extraction method, meanwhile for TCA extraction method protein expressed highly at range of Mw 50-75 kDa as resolved using SDS-PAGE. Through 2D-PAGE, protein spotted on the gel highly expressed at range of Mw 8 kDa and 30 kDa for halal chicken samples but not highly expressed for non-halal chicken samples. Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35039/1/Nur%20Syafinaz%20Aqmal%2024%20pgs.pdf text en http://ir.unimas.my/id/eprint/35039/4/Nur%20Syafinaz%20Aqmal%20ft.pdf Nur Syafinaz Aqmal, Paijo (2018) 2D PAGE analysis of chicken samples from different slaughtering process. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Nur Syafinaz Aqmal, Paijo
2D PAGE analysis of chicken samples from different slaughtering process
description This study investigated the expression of protein in chicken meat that assumed to be influenced by variations of the method of slaughtering. There were two types of samples that depend on the method of slaughtering had been examined. Samples are: (i) Severing the trachea, oesophagus and carotid arteries and jugular veins which are halal method, and (ii) Cut directly the neck of the chicken without considering the veins which are non halal method. Proteins in the sample had been extracted by two different extraction methods and quantified with Bradford assay to know the protein concentration. Protein expressed in higher amounts mostly found distributed at range of Mw 63-100 kDa for direct extraction method, meanwhile for TCA extraction method protein expressed highly at range of Mw 50-75 kDa as resolved using SDS-PAGE. Through 2D-PAGE, protein spotted on the gel highly expressed at range of Mw 8 kDa and 30 kDa for halal chicken samples but not highly expressed for non-halal chicken samples.
format Final Year Project Report
author Nur Syafinaz Aqmal, Paijo
author_facet Nur Syafinaz Aqmal, Paijo
author_sort Nur Syafinaz Aqmal, Paijo
title 2D PAGE analysis of chicken samples from different slaughtering process
title_short 2D PAGE analysis of chicken samples from different slaughtering process
title_full 2D PAGE analysis of chicken samples from different slaughtering process
title_fullStr 2D PAGE analysis of chicken samples from different slaughtering process
title_full_unstemmed 2D PAGE analysis of chicken samples from different slaughtering process
title_sort 2d page analysis of chicken samples from different slaughtering process
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/35039/1/Nur%20Syafinaz%20Aqmal%2024%20pgs.pdf
http://ir.unimas.my/id/eprint/35039/4/Nur%20Syafinaz%20Aqmal%20ft.pdf
http://ir.unimas.my/id/eprint/35039/
_version_ 1789945482959650816
score 13.211869

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