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Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells

The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of c...

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Main Authors: Norsharina, Ismail, Akhtar, Muhammad Nadeem, Maznah, Ismail, Seema, Zareen, Syed Adnan, Ali Shah, Nordin, Hj Lajis, Saiful Nizam, Tajuddin
Format: Article
Language:English
Published: Taylor & Francis 2015
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf
http://umpir.ump.edu.my/id/eprint/16897/
http://www.tandfonline.com/doi/abs/10.1080/14786419.2014.985676
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spelling my.ump.umpir.168972018-04-19T02:34:26Z http://umpir.ump.edu.my/id/eprint/16897/ Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells Norsharina, Ismail Akhtar, Muhammad Nadeem Maznah, Ismail Seema, Zareen Syed Adnan, Ali Shah Nordin, Hj Lajis Saiful Nizam, Tajuddin Q Science (General) The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. Taylor & Francis 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf Norsharina, Ismail and Akhtar, Muhammad Nadeem and Maznah, Ismail and Seema, Zareen and Syed Adnan, Ali Shah and Nordin, Hj Lajis and Saiful Nizam, Tajuddin (2015) Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells. Natural Product Research: Formerly Natural Product Letters, 29 (16). pp. 1571-1574. ISSN 1478-6419 (print); 1478-6427 (online) http://www.tandfonline.com/doi/abs/10.1080/14786419.2014.985676 DOI: 10.1080/14786419.2014.985676
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Norsharina, Ismail
Akhtar, Muhammad Nadeem
Maznah, Ismail
Seema, Zareen
Syed Adnan, Ali Shah
Nordin, Hj Lajis
Saiful Nizam, Tajuddin
Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
description The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects.
format Article
author Norsharina, Ismail
Akhtar, Muhammad Nadeem
Maznah, Ismail
Seema, Zareen
Syed Adnan, Ali Shah
Nordin, Hj Lajis
Saiful Nizam, Tajuddin
author_facet Norsharina, Ismail
Akhtar, Muhammad Nadeem
Maznah, Ismail
Seema, Zareen
Syed Adnan, Ali Shah
Nordin, Hj Lajis
Saiful Nizam, Tajuddin
author_sort Norsharina, Ismail
title Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
title_short Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
title_full Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
title_fullStr Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
title_full_unstemmed Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
title_sort neuroprotective effect from stem bark extracts of knema laurina against h2o2-and aβ1-42-induced cell death in human sh-sy5y cells
publisher Taylor & Francis
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf
http://umpir.ump.edu.my/id/eprint/16897/
http://www.tandfonline.com/doi/abs/10.1080/14786419.2014.985676
_version_ 1643668037875793920
score 13.211869

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