Neuroprotective Effect from Stem Bark Extracts of Knema Laurina Against H2O2-and Aβ1-42-induced Cell Death in Human SH-SY5Y Cells
The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of c...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2015
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/16897/1/fist-2015-sharina-Neuroprotective%20effect1.pdf http://umpir.ump.edu.my/id/eprint/16897/ http://www.tandfonline.com/doi/abs/10.1080/14786419.2014.985676 |
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| Summary: | The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid b-peptide 1 –42 length (Ab1 – 42)-induced cell death in differentiated SH-SY5Y cells. Exposure of 250mM H2O2 or 20 mM Ab1 – 42 to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 mg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 mg/mL exerted neuroprotective effect on Ab1 – 42-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Ab1 – 42-induced cell death. Flavonoids (3–6) and b-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects. |
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