Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicate...
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my.iium.irep.37362021-07-14T01:45:11Z http://irep.iium.edu.my/3736/ Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah QD Chemistry TP248.13 Biotechnology A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with the experimental data. All the three parameters were statistically significant at p < 0.05. The validation experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen demand (COD) removal throughout the fermentation period. Elsevier B.V. 2011-06-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3736/1/MOLCAB_process_optimization_article.pdf Salihu, Aliyu and Alam, Md Zahangir and AbdulKarim, M. Ismail and Mohd. Salleh, Hamzah (2011) Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium. Journal of Molecular Catalysis B: Enzymatic, 72 ( ). pp. 187-192. ISSN 1381-1177 http://dx.doi.org/10.1016/j.molcatb.2011.06.004 doi:10.1016/j.molcatb.2011.06.004 |
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QD Chemistry TP248.13 Biotechnology Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
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A two-level full factorial design (FFD) was employed to determine the effects of process parameters on
lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed
a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with
the experimental data. All the three parameters were statistically significant at p < 0.05. The validation
experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen
demand (COD) removal throughout the fermentation period. |
format |
Article |
author |
Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah |
author_facet |
Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah |
author_sort |
Salihu, Aliyu |
title |
Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
title_short |
Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
title_full |
Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
title_fullStr |
Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
title_full_unstemmed |
Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
title_sort |
effect of process parameters on lipase production by candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium |
publisher |
Elsevier B.V. |
publishDate |
2011 |
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http://irep.iium.edu.my/3736/1/MOLCAB_process_optimization_article.pdf http://irep.iium.edu.my/3736/ http://dx.doi.org/10.1016/j.molcatb.2011.06.004 |
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