Effect of process parameters on lipase production by Candida cylindracea in stirred tank bioreactor using renewable palm oil mill effluent based medium
A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicate...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier B.V.
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3736/1/MOLCAB_process_optimization_article.pdf http://irep.iium.edu.my/3736/ http://dx.doi.org/10.1016/j.molcatb.2011.06.004 |
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| Summary: | A two-level full factorial design (FFD) was employed to determine the effects of process parameters on
lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME)-based medium. Ten experimental runs based on three parameters (temperature, agitation and aeration) as indicated by the FFD were carried out in a stirred-tank bioreactor. On statistical analysis of the results, the optimum temperature, aeration and agitation rates were found to be 30 ◦C, 1.0 vvm and 400 rpm respectively, with a maximum activity of 41.46 U/ml after 36 h of fermentation. Analysis of variance (ANOVA) showed
a high coefficient of determination (R2) value of 0.999, indicating a satisfactory fit of the model with
the experimental data. All the three parameters were statistically significant at p < 0.05. The validation
experiment also confirmed that apart from lipase production, there was an increase in chemical oxygen
demand (COD) removal throughout the fermentation period. |
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