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A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy

Seaweed or macroalgae contain an abundance of valuable bioactive compounds such as proteins, carotenoids, and phenolics and, consequently, present great commercial interest. The aim of this work is the study and screening of recovering the protein components from the green macroalgae species Ulva la...

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Main Authors: Darnis, Deny Susanti, Mohammad Nor, Normawaty, Bakhtiar, M. Taher, Aminudin, Nurul Iman, Shukor, Muhammad Idham
Format: Conference or Workshop Item
Language:English
English
English
Published: 2022
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/100480/2/GENERAL%20SCHEDULE%20PROGRAM%20IC3PE%202022.pdf
http://irep.iium.edu.my/100480/3/Muhammad%20Idham%20-%20IC3PE%202022.pdf
http://irep.iium.edu.my/100480/19/Program%20Book%204th%20IC3PE%202022%20fix.pdf
http://irep.iium.edu.my/100480/
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spelling my.iium.irep.1004802022-10-13T06:33:30Z http://irep.iium.edu.my/100480/ A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy Darnis, Deny Susanti Mohammad Nor, Normawaty Bakhtiar, M. Taher Aminudin, Nurul Iman Shukor, Muhammad Idham QD Chemistry Seaweed or macroalgae contain an abundance of valuable bioactive compounds such as proteins, carotenoids, and phenolics and, consequently, present great commercial interest. The aim of this work is the study and screening of recovering the protein components from the green macroalgae species Ulva lactuca through conventional assisted extraction method by using a food grade extraction procedure. The effect of three operational conditions-namely, temperature (30–70 C), duration (1–3 h) and solute-to-solvent ratio (1-10 % w/v), was examined regarding the extracts’ yield (gravimetrically) using Kjeldahl analysis. Data analysis resulted in the optimal extraction conditions of 70 C, for 3 h with 10:100 (g/mL) solute-to-solvent ratio. The significant factors during the extraction procedure were identified using the Two-Level Factorial design. Solute-to-solvent ratio exhibit the significant effects with the optimum condition was noted at 10:100 (g/mL). The extraction duration and the extraction temperature somehow indicated a non-significant effect towards the protein yields. The experimental data and predicted results were considered comparable, and consequently, the corresponding regression models were sufficiently reliable for prediction. The protein fraction may be further concentrated and purified for use in food formulations. 2022-09-27 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/100480/2/GENERAL%20SCHEDULE%20PROGRAM%20IC3PE%202022.pdf application/pdf en http://irep.iium.edu.my/100480/3/Muhammad%20Idham%20-%20IC3PE%202022.pdf application/pdf en http://irep.iium.edu.my/100480/19/Program%20Book%204th%20IC3PE%202022%20fix.pdf Darnis, Deny Susanti and Mohammad Nor, Normawaty and Bakhtiar, M. Taher and Aminudin, Nurul Iman and Shukor, Muhammad Idham (2022) A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy. In: 4th International Conference on Chemistry, Chemical Process and Engineering (IC3PE) 2022, 27th September 2022, Jogjakarta, Indonesia. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic QD Chemistry
spellingShingle QD Chemistry
Darnis, Deny Susanti
Mohammad Nor, Normawaty
Bakhtiar, M. Taher
Aminudin, Nurul Iman
Shukor, Muhammad Idham
A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy
description Seaweed or macroalgae contain an abundance of valuable bioactive compounds such as proteins, carotenoids, and phenolics and, consequently, present great commercial interest. The aim of this work is the study and screening of recovering the protein components from the green macroalgae species Ulva lactuca through conventional assisted extraction method by using a food grade extraction procedure. The effect of three operational conditions-namely, temperature (30–70 C), duration (1–3 h) and solute-to-solvent ratio (1-10 % w/v), was examined regarding the extracts’ yield (gravimetrically) using Kjeldahl analysis. Data analysis resulted in the optimal extraction conditions of 70 C, for 3 h with 10:100 (g/mL) solute-to-solvent ratio. The significant factors during the extraction procedure were identified using the Two-Level Factorial design. Solute-to-solvent ratio exhibit the significant effects with the optimum condition was noted at 10:100 (g/mL). The extraction duration and the extraction temperature somehow indicated a non-significant effect towards the protein yields. The experimental data and predicted results were considered comparable, and consequently, the corresponding regression models were sufficiently reliable for prediction. The protein fraction may be further concentrated and purified for use in food formulations.
format Conference or Workshop Item
author Darnis, Deny Susanti
Mohammad Nor, Normawaty
Bakhtiar, M. Taher
Aminudin, Nurul Iman
Shukor, Muhammad Idham
author_facet Darnis, Deny Susanti
Mohammad Nor, Normawaty
Bakhtiar, M. Taher
Aminudin, Nurul Iman
Shukor, Muhammad Idham
author_sort Darnis, Deny Susanti
title A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy
title_short A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy
title_full A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy
title_fullStr A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy
title_full_unstemmed A comparative study of protein-rich extract using food grade extraction procedure from marine algae, Ulva Lactuca (Chlorophyta): screening through a two-level factorial experimental design strategy
title_sort comparative study of protein-rich extract using food grade extraction procedure from marine algae, ulva lactuca (chlorophyta): screening through a two-level factorial experimental design strategy
publishDate 2022
url http://irep.iium.edu.my/100480/2/GENERAL%20SCHEDULE%20PROGRAM%20IC3PE%202022.pdf
http://irep.iium.edu.my/100480/3/Muhammad%20Idham%20-%20IC3PE%202022.pdf
http://irep.iium.edu.my/100480/19/Program%20Book%204th%20IC3PE%202022%20fix.pdf
http://irep.iium.edu.my/100480/
_version_ 1748180257709490176
score 13.211869

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