DNA repair at the gene level in trichothiodystrophy cells
Cells from trichothiodystrophy (TTD) group 3 patients are non-hypersensitive to uv irradiation. These cells perform normal genome repair of pyrimidine dimers but fail to excise 6-4 photoproducts and concomitantly, are unable to restore RNA synthesis levels following uv irradiation. This pointed to a...
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| Format: | Article |
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Universiti Kebangsaan Malaysia
2000
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| Online Access: | http://journalarticle.ukm.my/3779/ http://www.ukm.my/jsm/english_journals/vol29_2000/vol29_00page19-31.html |
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| Summary: | Cells from trichothiodystrophy (TTD) group 3 patients are non-hypersensitive to uv irradiation. These cells perform normal genome repair of pyrimidine dimers but fail to excise 6-4 photoproducts and concomitantly, are unable to restore RNA synthesis levels following uv irradiation. This pointed to a defect in gene-specific repair and this study was undertaken to examine repair of dimers at the gene-level. The results indicated a defect in gene-specific repair of dimers at early times post-irradiation due to the inability of the cells to repair dimers from the transcribed strand of the active gene. These findings indicate that TTD group 3 cells are unable to remove DNA lesions preferentially and this might contribute to other inherent symptoms of these patients. |
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