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Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium

The aim of this study was to determine the effect of Fe3+ concentration (0.35, 4.89, 9.44 and 13.99 mg/L) on the growth, protein content and total phenolic content of Chlorella sp. The Chlorella sp. cells were grown at 51% relative humidity, 25°C ± 2 under continuous illumination at 36 W irradiance...

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Main Authors: Dian Ariani,, Orasa Suriyaphan,, Nittaya Chaiyanate,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2011
Online Access:http://journalarticle.ukm.my/2396/1/11_Dian.pdf
http://journalarticle.ukm.my/2396/
http://www.ukm.my/jsm/
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spelling my-ukm.journal.23962016-12-14T06:31:30Z http://journalarticle.ukm.my/2396/ Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium Dian Ariani, Orasa Suriyaphan, Nittaya Chaiyanate, The aim of this study was to determine the effect of Fe3+ concentration (0.35, 4.89, 9.44 and 13.99 mg/L) on the growth, protein content and total phenolic content of Chlorella sp. The Chlorella sp. cells were grown at 51% relative humidity, 25°C ± 2 under continuous illumination at 36 W irradiance supplied by day-light fluorescent lamp, and agitated by bubbling at a flow rate 2.7 m/s2. Samples were collected every 2 days over 21 days of the cultivation period to estimate the growth of Chlorella sp. Protein and total phenolic content of samples were determined on phase 7th, 14th, and 21st day of cultivation. Statistical analysis showed that there were significant differences (p<0.05) on growth, protein content and total phenolic content of Chlorella sp. at different iron concentrations. These differences could be related to specific differences in the cell metabolism. Protein content (8.34 mg/g dry weight), total phenolic content (8.70 mgGAE/g dry weight), cell number (1.03 × 107 cell/mL) and the specific growth rate (μ) of Chlorella sp. (1.85/day) were highest at the lowest Fe3+ concentration (0.35 mg/L). Universiti Kebangsaan Malaysia 2011-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2396/1/11_Dian.pdf Dian Ariani, and Orasa Suriyaphan, and Nittaya Chaiyanate, (2011) Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium. Sains Malaysiana, 40 (4). pp. 353-358. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The aim of this study was to determine the effect of Fe3+ concentration (0.35, 4.89, 9.44 and 13.99 mg/L) on the growth, protein content and total phenolic content of Chlorella sp. The Chlorella sp. cells were grown at 51% relative humidity, 25°C ± 2 under continuous illumination at 36 W irradiance supplied by day-light fluorescent lamp, and agitated by bubbling at a flow rate 2.7 m/s2. Samples were collected every 2 days over 21 days of the cultivation period to estimate the growth of Chlorella sp. Protein and total phenolic content of samples were determined on phase 7th, 14th, and 21st day of cultivation. Statistical analysis showed that there were significant differences (p<0.05) on growth, protein content and total phenolic content of Chlorella sp. at different iron concentrations. These differences could be related to specific differences in the cell metabolism. Protein content (8.34 mg/g dry weight), total phenolic content (8.70 mgGAE/g dry weight), cell number (1.03 × 107 cell/mL) and the specific growth rate (μ) of Chlorella sp. (1.85/day) were highest at the lowest Fe3+ concentration (0.35 mg/L).
format Article
author Dian Ariani,
Orasa Suriyaphan,
Nittaya Chaiyanate,
spellingShingle Dian Ariani,
Orasa Suriyaphan,
Nittaya Chaiyanate,
Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
author_facet Dian Ariani,
Orasa Suriyaphan,
Nittaya Chaiyanate,
author_sort Dian Ariani,
title Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
title_short Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
title_full Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
title_fullStr Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
title_full_unstemmed Effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
title_sort effect of iron concentration on growth, protein content and total phenolic content of chlorella sp. cultured in basal medium
publisher Universiti Kebangsaan Malaysia
publishDate 2011
url http://journalarticle.ukm.my/2396/1/11_Dian.pdf
http://journalarticle.ukm.my/2396/
http://www.ukm.my/jsm/
_version_ 1643735366038978560
score 13.211869

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