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Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer

Xanthomonas spp. synthesize a complex exopolysaccharide called xanthan gum, which has significant commercial value. Xanthomonas-infected vegetables exhibiting typical spot symptoms were used in this study. Isolation was done by streaking the sample suspension on the Yeast Malt agar. A colony that re...

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Main Authors: Khanom Simarani,, Nur Izlin Shafinaz Bokhari,, Haziqah Mohd Saleh,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/21441/1/M%2024.pdf
http://journalarticle.ukm.my/21441/
https://jms.mabjournal.com/index.php/mab/index
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spelling my-ukm.journal.214412023-04-04T01:35:04Z http://journalarticle.ukm.my/21441/ Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer Khanom Simarani, Nur Izlin Shafinaz Bokhari, Haziqah Mohd Saleh, Xanthomonas spp. synthesize a complex exopolysaccharide called xanthan gum, which has significant commercial value. Xanthomonas-infected vegetables exhibiting typical spot symptoms were used in this study. Isolation was done by streaking the sample suspension on the Yeast Malt agar. A colony that resembled Xanthomonas spp. was purified before a simple phenotypic test and identified using BIOLOG and 16 rRNA sequencing. The screening was done based on their performance by fermentation in a shake flask, under controlled conditions. The yield and viscosity of xanthan gum produced from each bacterium were compared to Xanthomonas campestris PV. campestris strain ATCC33913. There were 411 wild types of Xanthomonas spp. successfully isolated and ten strains were selected for xanthan gum evaluation. The results showed, the xanthan production (g/L) varied from (1.57 - 8.24) with the yield of xanthan from 0.64 to 4.71 g/g biomass. Strain C206 produced the highest xanthan gum concentration (8.24±0.20 g/L) compared to others and the control strain, ATCC 33913 (2.27±0.10g/L). The highest yield of xanthan 4.71±0.18 (g/g biomass) was produced by strain C298 followed by strain C279 with 3.88±0.04 (g/g biomass). From our investigation, the production and yield of xanthan gum and the viscosity of the polymers were significantly dependent on the bacterial strain. Based on the stable viscosity and yield of xanthan produced, X. campestris C279 was selected for further studies on product optimization. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21441/1/M%2024.pdf Khanom Simarani, and Nur Izlin Shafinaz Bokhari, and Haziqah Mohd Saleh, (2022) Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer. Malaysian Applied Biology, 51 (4). pp. 221-227. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Xanthomonas spp. synthesize a complex exopolysaccharide called xanthan gum, which has significant commercial value. Xanthomonas-infected vegetables exhibiting typical spot symptoms were used in this study. Isolation was done by streaking the sample suspension on the Yeast Malt agar. A colony that resembled Xanthomonas spp. was purified before a simple phenotypic test and identified using BIOLOG and 16 rRNA sequencing. The screening was done based on their performance by fermentation in a shake flask, under controlled conditions. The yield and viscosity of xanthan gum produced from each bacterium were compared to Xanthomonas campestris PV. campestris strain ATCC33913. There were 411 wild types of Xanthomonas spp. successfully isolated and ten strains were selected for xanthan gum evaluation. The results showed, the xanthan production (g/L) varied from (1.57 - 8.24) with the yield of xanthan from 0.64 to 4.71 g/g biomass. Strain C206 produced the highest xanthan gum concentration (8.24±0.20 g/L) compared to others and the control strain, ATCC 33913 (2.27±0.10g/L). The highest yield of xanthan 4.71±0.18 (g/g biomass) was produced by strain C298 followed by strain C279 with 3.88±0.04 (g/g biomass). From our investigation, the production and yield of xanthan gum and the viscosity of the polymers were significantly dependent on the bacterial strain. Based on the stable viscosity and yield of xanthan produced, X. campestris C279 was selected for further studies on product optimization.
format Article
author Khanom Simarani,
Nur Izlin Shafinaz Bokhari,
Haziqah Mohd Saleh,
spellingShingle Khanom Simarani,
Nur Izlin Shafinaz Bokhari,
Haziqah Mohd Saleh,
Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
author_facet Khanom Simarani,
Nur Izlin Shafinaz Bokhari,
Haziqah Mohd Saleh,
author_sort Khanom Simarani,
title Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
title_short Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
title_full Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
title_fullStr Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
title_full_unstemmed Characterization and identification of Xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
title_sort characterization and identification of xanthomonas spp. isolated from infected brassicaceae and selection of potential xanthan gum producer
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/21441/1/M%2024.pdf
http://journalarticle.ukm.my/21441/
https://jms.mabjournal.com/index.php/mab/index
_version_ 1762393392814227456
score 13.211869

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