Biophysical properties of DNA in hydrated ionic liquids
The biophysical properties and behavior of natural calf thymus DNA in hydrated 1-ethyl-3-butylimidazolium bromide ionic liquid (C2bimBr) have been studied using spectroscopy technique. The effect of ionic liquid concentration and temperature towards the duplex B-DNA conformation were determined. The...
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| 主要な著者: | , , , |
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| フォーマット: | Conference or Workshop Item |
| 出版事項: |
American Institute of Physics Inc.
2016
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| オンライン･アクセス: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006056274&doi=10.1063%2f1.4968137&partnerID=40&md5=79f5dc44cef8ceb3dd6c71b18cd87b6b http://eprints.utp.edu.my/30593/ |
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| 要約: | The biophysical properties and behavior of natural calf thymus DNA in hydrated 1-ethyl-3-butylimidazolium bromide ionic liquid (C2bimBr) have been studied using spectroscopy technique. The effect of ionic liquid concentration and temperature towards the duplex B-DNA conformation were determined. The presence of ionic liquid causes higher duplex DNA stability with the DNA melting temperature of â�¼56°C without any addition of buffer solutions. The electrostatic attraction between ionic liquid's cation and DNA phosphates groups was found play a main role in stabilizing native DNA structure. Understanding of the biophysical properties of DNA in this ionic media could be used as a platform for future development of specific solvent for nucleic acid nanotechnology. © 2016 Author(s). |
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