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Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil

Flavonoid group is a versatile compound as it can be used in the food, pharmaceutical, and urea fertilizer industry. Five main factors have been consistently studied for optimization, which are solid-to-solvent ratios, solvent-to-solvent ratios, the temperature of extraction medium, exposure time, a...

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Main Authors: Roslan, H., Mansor, N., Yahya, W.Z.N., Affendi, N.M.N.
Format: Conference or Workshop Item
Published: IOP Publishing Ltd 2020
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092211885&doi=10.1088%2f1755-1315%2f494%2f1%2f012012&partnerID=40&md5=1897cc40796194607a70f9fe357c6170
http://eprints.utp.edu.my/30033/
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spelling my.utp.eprints.300332022-03-25T03:21:58Z Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil Roslan, H. Mansor, N. Yahya, W.Z.N. Affendi, N.M.N. Flavonoid group is a versatile compound as it can be used in the food, pharmaceutical, and urea fertilizer industry. Five main factors have been consistently studied for optimization, which are solid-to-solvent ratios, solvent-to-solvent ratios, the temperature of extraction medium, exposure time, and equipment power intensity. Factors Solid-to-Solvent Ratio and Time showed the greatest effects on the extraction. Response surface methodology is used to determine the best conditions for flavonoid extraction from guava leaves using an ultrasonic bath. There exist incongruencies between researchers in determining which solvent is best used for flavonoid extraction. Experimentally, the highest total flavonoid content (TFC) tested using Fe (II) colorimetry was 50.44 mg of Quercetin-equivalent per gram of dry weight leaves (mg QE/g d.w.) using 1:1:1 ratio of Water-Ethanol-Methanol (WEM), a first tri-solvent based extraction for flavonoid. Further testing via urea colorimetry shows that diluted compound of 8 of flavonoid extract can reduce urea transformation starting Day 3 of soil sampling but its performance is not at par with the commercial n-Butylphosphorothioic Triadmide (NBPT) compared in this study. Nevertheless, the effect shows a positive outlook on the extract's potential as a urease inhibitor. © Published under licence by IOP Publishing Ltd. IOP Publishing Ltd 2020 Conference or Workshop Item NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092211885&doi=10.1088%2f1755-1315%2f494%2f1%2f012012&partnerID=40&md5=1897cc40796194607a70f9fe357c6170 Roslan, H. and Mansor, N. and Yahya, W.Z.N. and Affendi, N.M.N. (2020) Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil. In: UNSPECIFIED. http://eprints.utp.edu.my/30033/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
description Flavonoid group is a versatile compound as it can be used in the food, pharmaceutical, and urea fertilizer industry. Five main factors have been consistently studied for optimization, which are solid-to-solvent ratios, solvent-to-solvent ratios, the temperature of extraction medium, exposure time, and equipment power intensity. Factors Solid-to-Solvent Ratio and Time showed the greatest effects on the extraction. Response surface methodology is used to determine the best conditions for flavonoid extraction from guava leaves using an ultrasonic bath. There exist incongruencies between researchers in determining which solvent is best used for flavonoid extraction. Experimentally, the highest total flavonoid content (TFC) tested using Fe (II) colorimetry was 50.44 mg of Quercetin-equivalent per gram of dry weight leaves (mg QE/g d.w.) using 1:1:1 ratio of Water-Ethanol-Methanol (WEM), a first tri-solvent based extraction for flavonoid. Further testing via urea colorimetry shows that diluted compound of 8 of flavonoid extract can reduce urea transformation starting Day 3 of soil sampling but its performance is not at par with the commercial n-Butylphosphorothioic Triadmide (NBPT) compared in this study. Nevertheless, the effect shows a positive outlook on the extract's potential as a urease inhibitor. © Published under licence by IOP Publishing Ltd.
format Conference or Workshop Item
author Roslan, H.
Mansor, N.
Yahya, W.Z.N.
Affendi, N.M.N.
spellingShingle Roslan, H.
Mansor, N.
Yahya, W.Z.N.
Affendi, N.M.N.
Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil
author_facet Roslan, H.
Mansor, N.
Yahya, W.Z.N.
Affendi, N.M.N.
author_sort Roslan, H.
title Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil
title_short Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil
title_full Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil
title_fullStr Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil
title_full_unstemmed Optimization of Flavonoid Extraction from Guava Leaves for Application in Reducing Urea Transformation in Soil
title_sort optimization of flavonoid extraction from guava leaves for application in reducing urea transformation in soil
publisher IOP Publishing Ltd
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092211885&doi=10.1088%2f1755-1315%2f494%2f1%2f012012&partnerID=40&md5=1897cc40796194607a70f9fe357c6170
http://eprints.utp.edu.my/30033/
_version_ 1738657050368409600
score 13.211869

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