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Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour

Six different solvents were used as extraction medium (water, methanol, ethanol, acidified methanol, benzene and acetone) to check their phenolics extraction efficacy from flour of two rye cultivars. Rye extracts with different solvents were further analyzed for the estimation of phytochemicals and...

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Main Authors: Kaur, P., Sandhu, K. S., Bangar, S. P., Purewal, S. S., Kaur, M., Ilyas, R. A., Asyraf, M. R. M., Razman, M. R.
Format: Article
Language:English
Published: MDPI 2021
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/96585/1/RushdanAhmadIlyas2021_UnravelingtheBioactiveProfile.pdf
http://eprints.utm.my/id/eprint/96585/
http://dx.doi.org/10.3390/antiox10081214
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spelling my.utm.965852022-07-28T07:07:35Z http://eprints.utm.my/id/eprint/96585/ Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour Kaur, P. Sandhu, K. S. Bangar, S. P. Purewal, S. S. Kaur, M. Ilyas, R. A. Asyraf, M. R. M. Razman, M. R. TP Chemical technology Six different solvents were used as extraction medium (water, methanol, ethanol, acidified methanol, benzene and acetone) to check their phenolics extraction efficacy from flour of two rye cultivars. Rye extracts with different solvents were further analyzed for the estimation of phytochemicals and antioxidant properties. Different tests (TPC, TAC, DPPH, FRAP, ABTS, RPA and CTC) were performed to check the antioxidant properties and tannin contents in extracts. A bioactive profile of a rye cultivar indicated the presence of total phenolic compounds (0.08-2.62 mg GAE/g), total antioxidant capacity (0.9-6.8 mg AAE/g) and condensed tannin content (4.24-9.28 mg CE/100 g). HPLC was done to check phenolics in rye extract with the best solvent (water), which indicated the presence of Catechol (91.1-120.4 mg/100 g), resorcinol (52-70.3 mg/100 g), vanillin (1.3-5.5 mg/100 g), ferulic acid (1.4-1.5 mg/100 g), quercetin (4.6-4.67 mg/100 g) and benzoic acid (5.3 mg/100 g) in rye extracts. The presence of DNA damage protection potential in rye extracts indicates its medicinal importance. Rye flour could be utilized in the preparation of antioxidant-rich health-benefiting food products. MDPI 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/96585/1/RushdanAhmadIlyas2021_UnravelingtheBioactiveProfile.pdf Kaur, P. and Sandhu, K. S. and Bangar, S. P. and Purewal, S. S. and Kaur, M. and Ilyas, R. A. and Asyraf, M. R. M. and Razman, M. R. (2021) Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour. Antioxidants, 10 (8). p. 1214. ISSN 2076-3921 http://dx.doi.org/10.3390/antiox10081214 DOI: 10.3390/antiox10081214
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Kaur, P.
Sandhu, K. S.
Bangar, S. P.
Purewal, S. S.
Kaur, M.
Ilyas, R. A.
Asyraf, M. R. M.
Razman, M. R.
Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour
description Six different solvents were used as extraction medium (water, methanol, ethanol, acidified methanol, benzene and acetone) to check their phenolics extraction efficacy from flour of two rye cultivars. Rye extracts with different solvents were further analyzed for the estimation of phytochemicals and antioxidant properties. Different tests (TPC, TAC, DPPH, FRAP, ABTS, RPA and CTC) were performed to check the antioxidant properties and tannin contents in extracts. A bioactive profile of a rye cultivar indicated the presence of total phenolic compounds (0.08-2.62 mg GAE/g), total antioxidant capacity (0.9-6.8 mg AAE/g) and condensed tannin content (4.24-9.28 mg CE/100 g). HPLC was done to check phenolics in rye extract with the best solvent (water), which indicated the presence of Catechol (91.1-120.4 mg/100 g), resorcinol (52-70.3 mg/100 g), vanillin (1.3-5.5 mg/100 g), ferulic acid (1.4-1.5 mg/100 g), quercetin (4.6-4.67 mg/100 g) and benzoic acid (5.3 mg/100 g) in rye extracts. The presence of DNA damage protection potential in rye extracts indicates its medicinal importance. Rye flour could be utilized in the preparation of antioxidant-rich health-benefiting food products.
format Article
author Kaur, P.
Sandhu, K. S.
Bangar, S. P.
Purewal, S. S.
Kaur, M.
Ilyas, R. A.
Asyraf, M. R. M.
Razman, M. R.
author_facet Kaur, P.
Sandhu, K. S.
Bangar, S. P.
Purewal, S. S.
Kaur, M.
Ilyas, R. A.
Asyraf, M. R. M.
Razman, M. R.
author_sort Kaur, P.
title Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour
title_short Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour
title_full Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour
title_fullStr Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour
title_full_unstemmed Unraveling the bioactive profile, antioxidant and DNA damage protection potential of rye (secale cereale) flour
title_sort unraveling the bioactive profile, antioxidant and dna damage protection potential of rye (secale cereale) flour
publisher MDPI
publishDate 2021
url http://eprints.utm.my/id/eprint/96585/1/RushdanAhmadIlyas2021_UnravelingtheBioactiveProfile.pdf
http://eprints.utm.my/id/eprint/96585/
http://dx.doi.org/10.3390/antiox10081214
_version_ 1739828100565303296
score 13.211869

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