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iPS cell transplantation in a mouse model – A semiquantitative assessment

This study was carried out to determine the effectiveness of induced pluripotent stem (iPS) cell-transplantation as a therapy on wound healing using a splinted wound mouse model. Eighteen clinically healthy female mice were treated with 1μg/g of dexamethasone intramuscular injection once a day for t...

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Main Authors:	Ong, Patrick Yit Han, Hashim, Zanariah, Abd. Wahid, Mohd. Effendy, Bee, Diana Lan Ong, Bakkery, Nur Adibah, Md. Zin, Reena Rahayu, Tizen Liam, Nur Maya Sabrina, Abd. Rahman, Faridah, Mustangin, Muaatamarulain, Hajri, Norazwin, Abdul Majid, Fadzilah Adibah
Format:	Article
Language:	English
Published:	Penerbit UTM Press 2020
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/93067/1/ZanariahHashim2020_iPSCellTransplantationInAMouseModel.pdf http://eprints.utm.my/id/eprint/93067/ http://dx.doi.org/10.11113/mjfas.v16n6.1875
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spelling	my.utm.930672021-11-07T05:59:50Z http://eprints.utm.my/id/eprint/93067/ iPS cell transplantation in a mouse model – A semiquantitative assessment Ong, Patrick Yit Han Hashim, Zanariah Abd. Wahid, Mohd. Effendy Bee, Diana Lan Ong Bakkery, Nur Adibah Md. Zin, Reena Rahayu Tizen Liam, Nur Maya Sabrina Abd. Rahman, Faridah Mustangin, Muaatamarulain Hajri, Norazwin Abdul Majid, Fadzilah Adibah TP Chemical technology This study was carried out to determine the effectiveness of induced pluripotent stem (iPS) cell-transplantation as a therapy on wound healing using a splinted wound mouse model. Eighteen clinically healthy female mice were treated with 1μg/g of dexamethasone intramuscular injection once a day for three consecutive days to induce immunosuppression. Under anaesthesia, two sterile wounds were made on shaved backs of each mouse via biopsy punch. With a gap of 20 mm in-between, six injections were delivered once, around the two wounds before the wounds were adhered with splints and wound dressing. The mice were divided into two groups; Group A was given 7 × 105 iPS cells in each injection, and while Group B were injected with 0.9% sodium chloride instead. Wound closure rates were determined through timed scaled photography and analyses with GNU Image Manipulation Program. Three mice from each group were euthanised every 7 days post-wounding, immediately after which wound beds and blood were harvested. Wound beds were fixed, processed, blocked, and sectioned. Sections were stained with H&E; Masson's Trichrome; and immunolabeled for CD31 and CD68; and then examined under a compound microscope subjected to a scoring scheme. From this semiquantitative assessment, Group A sections scored better in angiogenesis on day 7 (p = 0.057), a vital process in the proliferation stage, and hypodermis regeneration on day 21 (p = 0.006), which suggests the wound healing is complete. Together, these findings suggest Group A was ahead in the process of wound healing; although results from lymphocyte count, fibroblast count, granulation tissue, collagen, and wound closure were not statistically significant. Penerbit UTM Press 2020 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/93067/1/ZanariahHashim2020_iPSCellTransplantationInAMouseModel.pdf Ong, Patrick Yit Han and Hashim, Zanariah and Abd. Wahid, Mohd. Effendy and Bee, Diana Lan Ong and Bakkery, Nur Adibah and Md. Zin, Reena Rahayu and Tizen Liam, Nur Maya Sabrina and Abd. Rahman, Faridah and Mustangin, Muaatamarulain and Hajri, Norazwin and Abdul Majid, Fadzilah Adibah (2020) iPS cell transplantation in a mouse model – A semiquantitative assessment. Malaysian Journal of Fundamental and Applied Sciences, 16 (6). pp. 641-648. ISSN 2289-599X http://dx.doi.org/10.11113/mjfas.v16n6.1875
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ong, Patrick Yit Han Hashim, Zanariah Abd. Wahid, Mohd. Effendy Bee, Diana Lan Ong Bakkery, Nur Adibah Md. Zin, Reena Rahayu Tizen Liam, Nur Maya Sabrina Abd. Rahman, Faridah Mustangin, Muaatamarulain Hajri, Norazwin Abdul Majid, Fadzilah Adibah iPS cell transplantation in a mouse model – A semiquantitative assessment
description	This study was carried out to determine the effectiveness of induced pluripotent stem (iPS) cell-transplantation as a therapy on wound healing using a splinted wound mouse model. Eighteen clinically healthy female mice were treated with 1μg/g of dexamethasone intramuscular injection once a day for three consecutive days to induce immunosuppression. Under anaesthesia, two sterile wounds were made on shaved backs of each mouse via biopsy punch. With a gap of 20 mm in-between, six injections were delivered once, around the two wounds before the wounds were adhered with splints and wound dressing. The mice were divided into two groups; Group A was given 7 × 105 iPS cells in each injection, and while Group B were injected with 0.9% sodium chloride instead. Wound closure rates were determined through timed scaled photography and analyses with GNU Image Manipulation Program. Three mice from each group were euthanised every 7 days post-wounding, immediately after which wound beds and blood were harvested. Wound beds were fixed, processed, blocked, and sectioned. Sections were stained with H&E; Masson's Trichrome; and immunolabeled for CD31 and CD68; and then examined under a compound microscope subjected to a scoring scheme. From this semiquantitative assessment, Group A sections scored better in angiogenesis on day 7 (p = 0.057), a vital process in the proliferation stage, and hypodermis regeneration on day 21 (p = 0.006), which suggests the wound healing is complete. Together, these findings suggest Group A was ahead in the process of wound healing; although results from lymphocyte count, fibroblast count, granulation tissue, collagen, and wound closure were not statistically significant.
format	Article
author	Ong, Patrick Yit Han Hashim, Zanariah Abd. Wahid, Mohd. Effendy Bee, Diana Lan Ong Bakkery, Nur Adibah Md. Zin, Reena Rahayu Tizen Liam, Nur Maya Sabrina Abd. Rahman, Faridah Mustangin, Muaatamarulain Hajri, Norazwin Abdul Majid, Fadzilah Adibah
author_facet	Ong, Patrick Yit Han Hashim, Zanariah Abd. Wahid, Mohd. Effendy Bee, Diana Lan Ong Bakkery, Nur Adibah Md. Zin, Reena Rahayu Tizen Liam, Nur Maya Sabrina Abd. Rahman, Faridah Mustangin, Muaatamarulain Hajri, Norazwin Abdul Majid, Fadzilah Adibah
author_sort	Ong, Patrick Yit Han
title	iPS cell transplantation in a mouse model – A semiquantitative assessment
title_short	iPS cell transplantation in a mouse model – A semiquantitative assessment
title_full	iPS cell transplantation in a mouse model – A semiquantitative assessment
title_fullStr	iPS cell transplantation in a mouse model – A semiquantitative assessment
title_full_unstemmed	iPS cell transplantation in a mouse model – A semiquantitative assessment
title_sort	ips cell transplantation in a mouse model – a semiquantitative assessment
publisher	Penerbit UTM Press
publishDate	2020
url	http://eprints.utm.my/id/eprint/93067/1/ZanariahHashim2020_iPSCellTransplantationInAMouseModel.pdf http://eprints.utm.my/id/eprint/93067/ http://dx.doi.org/10.11113/mjfas.v16n6.1875
_version_	1717093415119749120
score	13.211869

iPS cell transplantation in a mouse model – A semiquantitative assessment

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