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Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia

Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simple monomers. Amylase enzymes are widely used in various industrial fields such as textile, food, paper and other industries. Compared to other organisms, yeasts can produce enzymes more effectively and s...

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Main Authors: Risandi, A., Fuady, R., Sukmawati, D., Husna, S. N. A., Nurjayadi, M., Enshasy, H. E., Ridawati, R.
Format: Conference or Workshop Item
Language:English
Published: 2019
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/92700/1/DSukmawati2019_IsolationandScreeningofAmylolyticYeast.pdf
http://eprints.utm.my/id/eprint/92700/
http://dx.doi.org/10.1088/1742-6596/1402/3/033060
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spelling my.utm.927002021-10-28T10:09:58Z http://eprints.utm.my/id/eprint/92700/ Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia Risandi, A. Fuady, R. Sukmawati, D. Husna, S. N. A. Nurjayadi, M. Enshasy, H. E. Ridawati, R. TP Chemical technology Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simple monomers. Amylase enzymes are widely used in various industrial fields such as textile, food, paper and other industries. Compared to other organisms, yeasts can produce enzymes more effectively and safer for the environment. Amylolytic yeast can be isolated from flower substrates as it contains sugar for the very limited condition of yeast growth. This study aims to isolate, select and characterize amylolytic yeasts on the substrate of Paphiopedilum sp. from Bedugul Botanical Garden, Bali. Yeast isolation was carried out with direct and washing method, followed by screening conducted on YPSA medium with diffusion agar method. Results showed that 19 yeast isolates were obtained with characteristics of 73.9% white-mucoid, 21.05% cream-mucoid and 5.26% light flesh-mucoid. Screening results showed that 10 isolates which coded by P1, P4, P6, P10, P11, P12, P13, P14, P15 and P19, were able to produce amylase enzyme. The potential yeast isolates in yielding amylase with P12 isolate codes had amylolytic index 0,45 mm. from this research, it can be found the symbiosis between yeasts and plants are happening in certain ways. 2019 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/92700/1/DSukmawati2019_IsolationandScreeningofAmylolyticYeast.pdf Risandi, A. and Fuady, R. and Sukmawati, D. and Husna, S. N. A. and Nurjayadi, M. and Enshasy, H. E. and Ridawati, R. (2019) Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia. In: 4th Annual Applied Science and Engineering Conference, AASEC 2019, 24 Apr 2019, Bali, Indonesia. http://dx.doi.org/10.1088/1742-6596/1402/3/033060
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Risandi, A.
Fuady, R.
Sukmawati, D.
Husna, S. N. A.
Nurjayadi, M.
Enshasy, H. E.
Ridawati, R.
Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia
description Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simple monomers. Amylase enzymes are widely used in various industrial fields such as textile, food, paper and other industries. Compared to other organisms, yeasts can produce enzymes more effectively and safer for the environment. Amylolytic yeast can be isolated from flower substrates as it contains sugar for the very limited condition of yeast growth. This study aims to isolate, select and characterize amylolytic yeasts on the substrate of Paphiopedilum sp. from Bedugul Botanical Garden, Bali. Yeast isolation was carried out with direct and washing method, followed by screening conducted on YPSA medium with diffusion agar method. Results showed that 19 yeast isolates were obtained with characteristics of 73.9% white-mucoid, 21.05% cream-mucoid and 5.26% light flesh-mucoid. Screening results showed that 10 isolates which coded by P1, P4, P6, P10, P11, P12, P13, P14, P15 and P19, were able to produce amylase enzyme. The potential yeast isolates in yielding amylase with P12 isolate codes had amylolytic index 0,45 mm. from this research, it can be found the symbiosis between yeasts and plants are happening in certain ways.
format Conference or Workshop Item
author Risandi, A.
Fuady, R.
Sukmawati, D.
Husna, S. N. A.
Nurjayadi, M.
Enshasy, H. E.
Ridawati, R.
author_facet Risandi, A.
Fuady, R.
Sukmawati, D.
Husna, S. N. A.
Nurjayadi, M.
Enshasy, H. E.
Ridawati, R.
author_sort Risandi, A.
title Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia
title_short Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia
title_full Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia
title_fullStr Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia
title_full_unstemmed Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia
title_sort isolation and screening of amylolytic yeast from paphiopedilum sp., originating from bedugul botanical garden, bali, indonesia
publishDate 2019
url http://eprints.utm.my/id/eprint/92700/1/DSukmawati2019_IsolationandScreeningofAmylolyticYeast.pdf
http://eprints.utm.my/id/eprint/92700/
http://dx.doi.org/10.1088/1742-6596/1402/3/033060
_version_ 1715189676930760704
score 13.211869

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