Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Elsevier BV
2006
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/9084/ http://dx.doi.org/10.1016/j.enzmictec.2005.06.006 |
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