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Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate

Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay...

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Main Authors: Fen, Lee Lih, Md.Illias, Rosli, Kamaruddin, Kamarulzaman, Maskat, Mohd Yusof, Hassan, Osman
Format: Article
Published: Elsevier BV 2006
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/9084/
http://dx.doi.org/10.1016/j.enzmictec.2005.06.006
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spelling my.utm.90842018-03-07T21:10:41Z http://eprints.utm.my/id/eprint/9084/ Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate Fen, Lee Lih Md.Illias, Rosli Kamaruddin, Kamarulzaman Maskat, Mohd Yusof Hassan, Osman TP Chemical technology Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay was performed on powdered (RBB-P) and colloidal form (RBB-C) of the dyed chitosan. Hydrolysis of chitosan was accompanied by the release of water-soluble dyed-hydrolysate, which can be monitored rapidly using microplate reader at 595 nm. The result obtained from this method was in agreement with the determination of reducing end by using the traditional, complex and time-consuming DNS method. The method is significantly rapid and more sensitive than DNS method. Elsevier BV 2006 Article PeerReviewed Fen, Lee Lih and Md.Illias, Rosli and Kamaruddin, Kamarulzaman and Maskat, Mohd Yusof and Hassan, Osman (2006) Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate. Enzyme and Microbial Technology, 38 (1-2). pp. 215-219. ISSN Enzyme and Microbial Technology http://dx.doi.org/10.1016/j.enzmictec.2005.06.006 10.1016/j.enzmictec.2005.06.006
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Fen, Lee Lih
Md.Illias, Rosli
Kamaruddin, Kamarulzaman
Maskat, Mohd Yusof
Hassan, Osman
Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
description Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50 °C. The chitosanase assay was performed on powdered (RBB-P) and colloidal form (RBB-C) of the dyed chitosan. Hydrolysis of chitosan was accompanied by the release of water-soluble dyed-hydrolysate, which can be monitored rapidly using microplate reader at 595 nm. The result obtained from this method was in agreement with the determination of reducing end by using the traditional, complex and time-consuming DNS method. The method is significantly rapid and more sensitive than DNS method.
format Article
author Fen, Lee Lih
Md.Illias, Rosli
Kamaruddin, Kamarulzaman
Maskat, Mohd Yusof
Hassan, Osman
author_facet Fen, Lee Lih
Md.Illias, Rosli
Kamaruddin, Kamarulzaman
Maskat, Mohd Yusof
Hassan, Osman
author_sort Fen, Lee Lih
title Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
title_short Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
title_full Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
title_fullStr Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
title_full_unstemmed Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate
title_sort development of rapid screening method for low-yielding chitosanase activity using remazol brilliant blue-chitosan as substrate
publisher Elsevier BV
publishDate 2006
url http://eprints.utm.my/id/eprint/9084/
http://dx.doi.org/10.1016/j.enzmictec.2005.06.006
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score 13.211869

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