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Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation

The engineering, characterization, and application of dual-functional delivery vehicle "SPIONs-Br-FA" are reported. In this study, a citrate-coated SPIONs (superparamagnetic iron oxide nanoparticles) drug-delivery vehicle was conjugated with Br (bromelain), a phytotherapeutic anticancer ag...

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Main Authors: Nasiri, R., Almaki, J. H., Idris, A., Nasiri, M., Irfan, M., Abdul Majid, F. A., Nodeh, H. R., Hasham, R.
Format: Article
Language:English
Published: Royal Society of Chemistry 2017
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/75735/1/RozitaNasiri_TargetedDeliveryofBromelainusingDualMode.pdf
http://eprints.utm.my/id/eprint/75735/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027851879&doi=10.1039%2fc7ra06389j&partnerID=40&md5=a1baaef5c6d3f4a6d79e3223d125733d
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spelling my.utm.757352018-04-30T13:13:46Z http://eprints.utm.my/id/eprint/75735/ Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation Nasiri, R. Almaki, J. H. Idris, A. Nasiri, M. Irfan, M. Abdul Majid, F. A. Nodeh, H. R. Hasham, R. TP Chemical technology The engineering, characterization, and application of dual-functional delivery vehicle "SPIONs-Br-FA" are reported. In this study, a citrate-coated SPIONs (superparamagnetic iron oxide nanoparticles) drug-delivery vehicle was conjugated with Br (bromelain), a phytotherapeutic anticancer agent, and finally immobilized with FA (folic acid), as a targeting moiety to the FAR+, folate receptor positive, cancer cells. Then, in vitro compatibility tests were performed to confirm the biocompatibility of the engineered system. A cytotoxicity study was carried out, which showed a significant dose advantage with SPIONs-Br-FA in reducing the IC50 values of FAR+ cancer cells compared with neat Br. Through morphological alternation studies, it was disclosed that the SPIONs-Br-FA-treated cells had undergone apoptosis, since shrinkage as well as apoptotic bodies were obviously observed. We demonstrated that SPIONs-Br-FA was a good candidate to suppress the migration of the FAR+ cancer cells as well as to inhibit colony formation of the FAR+ cancer cells compared to SPIONs-Br. We found that the apoptosis percentage was sharply increased in the FAR+ cancer cells treated by SPIONs-Br-FA compared to those treated by neat Br. Moreover, the qualitative and quantitative biodistribution study performed on the vital organs and tumor indicated a significant tumor targetability of the SPIONs-FA. Next, we demonstrated the administration of SPIONs-Br-FA through the tail vein could reduce the tumor burden in 4T1-bearing mice and also increased their lifespan when compared with SPIONs-Br and neat Br at the same concentration of bromelain. In conclusion, the results indicated that the synthesized SPIONs-Br-FA is a promising tool in the field of biomedicine, particularly in cancer therapy. Royal Society of Chemistry 2017 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/75735/1/RozitaNasiri_TargetedDeliveryofBromelainusingDualMode.pdf Nasiri, R. and Almaki, J. H. and Idris, A. and Nasiri, M. and Irfan, M. and Abdul Majid, F. A. and Nodeh, H. R. and Hasham, R. (2017) Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation. RSC Advances, 7 (64). pp. 40074-40094. ISSN 2046-2069 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027851879&doi=10.1039%2fc7ra06389j&partnerID=40&md5=a1baaef5c6d3f4a6d79e3223d125733d
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nasiri, R.
Almaki, J. H.
Idris, A.
Nasiri, M.
Irfan, M.
Abdul Majid, F. A.
Nodeh, H. R.
Hasham, R.
Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
description The engineering, characterization, and application of dual-functional delivery vehicle "SPIONs-Br-FA" are reported. In this study, a citrate-coated SPIONs (superparamagnetic iron oxide nanoparticles) drug-delivery vehicle was conjugated with Br (bromelain), a phytotherapeutic anticancer agent, and finally immobilized with FA (folic acid), as a targeting moiety to the FAR+, folate receptor positive, cancer cells. Then, in vitro compatibility tests were performed to confirm the biocompatibility of the engineered system. A cytotoxicity study was carried out, which showed a significant dose advantage with SPIONs-Br-FA in reducing the IC50 values of FAR+ cancer cells compared with neat Br. Through morphological alternation studies, it was disclosed that the SPIONs-Br-FA-treated cells had undergone apoptosis, since shrinkage as well as apoptotic bodies were obviously observed. We demonstrated that SPIONs-Br-FA was a good candidate to suppress the migration of the FAR+ cancer cells as well as to inhibit colony formation of the FAR+ cancer cells compared to SPIONs-Br. We found that the apoptosis percentage was sharply increased in the FAR+ cancer cells treated by SPIONs-Br-FA compared to those treated by neat Br. Moreover, the qualitative and quantitative biodistribution study performed on the vital organs and tumor indicated a significant tumor targetability of the SPIONs-FA. Next, we demonstrated the administration of SPIONs-Br-FA through the tail vein could reduce the tumor burden in 4T1-bearing mice and also increased their lifespan when compared with SPIONs-Br and neat Br at the same concentration of bromelain. In conclusion, the results indicated that the synthesized SPIONs-Br-FA is a promising tool in the field of biomedicine, particularly in cancer therapy.
format Article
author Nasiri, R.
Almaki, J. H.
Idris, A.
Nasiri, M.
Irfan, M.
Abdul Majid, F. A.
Nodeh, H. R.
Hasham, R.
author_facet Nasiri, R.
Almaki, J. H.
Idris, A.
Nasiri, M.
Irfan, M.
Abdul Majid, F. A.
Nodeh, H. R.
Hasham, R.
author_sort Nasiri, R.
title Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
title_short Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
title_full Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
title_fullStr Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
title_full_unstemmed Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
title_sort targeted delivery of bromelain using dual mode nanoparticles: synthesis, physicochemical characterization,: in vitro and in vivo evaluation
publisher Royal Society of Chemistry
publishDate 2017
url http://eprints.utm.my/id/eprint/75735/1/RozitaNasiri_TargetedDeliveryofBromelainusingDualMode.pdf
http://eprints.utm.my/id/eprint/75735/
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027851879&doi=10.1039%2fc7ra06389j&partnerID=40&md5=a1baaef5c6d3f4a6d79e3223d125733d
_version_ 1643657146333659136
score 13.211869

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