Targeted delivery of bromelain using dual mode nanoparticles: Synthesis, physicochemical characterization,: In vitro and in vivo evaluation
The engineering, characterization, and application of dual-functional delivery vehicle "SPIONs-Br-FA" are reported. In this study, a citrate-coated SPIONs (superparamagnetic iron oxide nanoparticles) drug-delivery vehicle was conjugated with Br (bromelain), a phytotherapeutic anticancer ag...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royal Society of Chemistry
2017
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/75735/1/RozitaNasiri_TargetedDeliveryofBromelainusingDualMode.pdf http://eprints.utm.my/id/eprint/75735/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027851879&doi=10.1039%2fc7ra06389j&partnerID=40&md5=a1baaef5c6d3f4a6d79e3223d125733d |
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| Summary: | The engineering, characterization, and application of dual-functional delivery vehicle "SPIONs-Br-FA" are reported. In this study, a citrate-coated SPIONs (superparamagnetic iron oxide nanoparticles) drug-delivery vehicle was conjugated with Br (bromelain), a phytotherapeutic anticancer agent, and finally immobilized with FA (folic acid), as a targeting moiety to the FAR+, folate receptor positive, cancer cells. Then, in vitro compatibility tests were performed to confirm the biocompatibility of the engineered system. A cytotoxicity study was carried out, which showed a significant dose advantage with SPIONs-Br-FA in reducing the IC50 values of FAR+ cancer cells compared with neat Br. Through morphological alternation studies, it was disclosed that the SPIONs-Br-FA-treated cells had undergone apoptosis, since shrinkage as well as apoptotic bodies were obviously observed. We demonstrated that SPIONs-Br-FA was a good candidate to suppress the migration of the FAR+ cancer cells as well as to inhibit colony formation of the FAR+ cancer cells compared to SPIONs-Br. We found that the apoptosis percentage was sharply increased in the FAR+ cancer cells treated by SPIONs-Br-FA compared to those treated by neat Br. Moreover, the qualitative and quantitative biodistribution study performed on the vital organs and tumor indicated a significant tumor targetability of the SPIONs-FA. Next, we demonstrated the administration of SPIONs-Br-FA through the tail vein could reduce the tumor burden in 4T1-bearing mice and also increased their lifespan when compared with SPIONs-Br and neat Br at the same concentration of bromelain. In conclusion, the results indicated that the synthesized SPIONs-Br-FA is a promising tool in the field of biomedicine, particularly in cancer therapy. |
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