Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction.
Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the...
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2023
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| Online Access: | http://eprints.utm.my/107806/1/HeshamAliElEnshasy2023_ValidationoftheDetectionKitforPathogenicBacteriaSalmonella.pdf http://eprints.utm.my/107806/ http://dx.doi.org/10.1051/e3sconf/202340004009 |
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my.utm.1078062024-10-02T07:41:00Z http://eprints.utm.my/107806/ Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. Maulana, Irvan Nurjayadi, Muktiningsih Ulfi Rahma Efrianti, Ulfi Rahma Efrianti Azizah, Noer Pramudiyasih, Nabilla Alya Ratna Nur Kusumawati, Ratna Nur Kusumawati Maharanianska Azzahra, Maharanianska Azzahra Declan, Jefferson Lynford Gladys Indira Putri, Gladys Indira Putri Juliansyah, Dandi Akbar Ismaya Krisdawati, Ismaya Krisdawati Fera Kurniadewi, Fera Kurniadewi Irma Ratna Kartika, Irma Ratna Kartika Dalia Sukmawati, Dalia Sukmawati Lidwina Nastassya, Lidwina Nastassya Vira Saamia, Vira Saamia Saputro, Dwi Anna Oktaviani I Made Wiranatha, I Made Wiranatha El-Enshasy, Hesham Ali TP Chemical technology Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60oC and a standard DNA concentration of 50 ng/μL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to the standard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistent and reproducible data. 2023-07-03 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/107806/1/HeshamAliElEnshasy2023_ValidationoftheDetectionKitforPathogenicBacteriaSalmonella.pdf Maulana, Irvan and Nurjayadi, Muktiningsih and Ulfi Rahma Efrianti, Ulfi Rahma Efrianti and Azizah, Noer and Pramudiyasih, Nabilla Alya and Ratna Nur Kusumawati, Ratna Nur Kusumawati and Maharanianska Azzahra, Maharanianska Azzahra and Declan, Jefferson Lynford and Gladys Indira Putri, Gladys Indira Putri and Juliansyah, Dandi Akbar and Ismaya Krisdawati, Ismaya Krisdawati and Fera Kurniadewi, Fera Kurniadewi and Irma Ratna Kartika, Irma Ratna Kartika and Dalia Sukmawati, Dalia Sukmawati and Lidwina Nastassya, Lidwina Nastassya and Vira Saamia, Vira Saamia and Saputro, Dwi Anna Oktaviani and I Made Wiranatha, I Made Wiranatha and El-Enshasy, Hesham Ali (2023) Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. In: 1st International Conference on Sciences, Mathematics, and Education, ICoSMEd 2022, 13 October 2022 - 14 October 2022, Gorontalo, Sulawesi, Indonesia. http://dx.doi.org/10.1051/e3sconf/202340004009 |
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TP Chemical technology Maulana, Irvan Nurjayadi, Muktiningsih Ulfi Rahma Efrianti, Ulfi Rahma Efrianti Azizah, Noer Pramudiyasih, Nabilla Alya Ratna Nur Kusumawati, Ratna Nur Kusumawati Maharanianska Azzahra, Maharanianska Azzahra Declan, Jefferson Lynford Gladys Indira Putri, Gladys Indira Putri Juliansyah, Dandi Akbar Ismaya Krisdawati, Ismaya Krisdawati Fera Kurniadewi, Fera Kurniadewi Irma Ratna Kartika, Irma Ratna Kartika Dalia Sukmawati, Dalia Sukmawati Lidwina Nastassya, Lidwina Nastassya Vira Saamia, Vira Saamia Saputro, Dwi Anna Oktaviani I Made Wiranatha, I Made Wiranatha El-Enshasy, Hesham Ali Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| description |
Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60oC and a standard DNA concentration of 50 ng/μL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to the standard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistent and reproducible data. |
| format |
Conference or Workshop Item |
| author |
Maulana, Irvan Nurjayadi, Muktiningsih Ulfi Rahma Efrianti, Ulfi Rahma Efrianti Azizah, Noer Pramudiyasih, Nabilla Alya Ratna Nur Kusumawati, Ratna Nur Kusumawati Maharanianska Azzahra, Maharanianska Azzahra Declan, Jefferson Lynford Gladys Indira Putri, Gladys Indira Putri Juliansyah, Dandi Akbar Ismaya Krisdawati, Ismaya Krisdawati Fera Kurniadewi, Fera Kurniadewi Irma Ratna Kartika, Irma Ratna Kartika Dalia Sukmawati, Dalia Sukmawati Lidwina Nastassya, Lidwina Nastassya Vira Saamia, Vira Saamia Saputro, Dwi Anna Oktaviani I Made Wiranatha, I Made Wiranatha El-Enshasy, Hesham Ali |
| author_facet |
Maulana, Irvan Nurjayadi, Muktiningsih Ulfi Rahma Efrianti, Ulfi Rahma Efrianti Azizah, Noer Pramudiyasih, Nabilla Alya Ratna Nur Kusumawati, Ratna Nur Kusumawati Maharanianska Azzahra, Maharanianska Azzahra Declan, Jefferson Lynford Gladys Indira Putri, Gladys Indira Putri Juliansyah, Dandi Akbar Ismaya Krisdawati, Ismaya Krisdawati Fera Kurniadewi, Fera Kurniadewi Irma Ratna Kartika, Irma Ratna Kartika Dalia Sukmawati, Dalia Sukmawati Lidwina Nastassya, Lidwina Nastassya Vira Saamia, Vira Saamia Saputro, Dwi Anna Oktaviani I Made Wiranatha, I Made Wiranatha El-Enshasy, Hesham Ali |
| author_sort |
Maulana, Irvan |
| title |
Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| title_short |
Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| title_full |
Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| title_fullStr |
Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| title_full_unstemmed |
Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| title_sort |
validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction. |
| publishDate |
2023 |
| url |
http://eprints.utm.my/107806/1/HeshamAliElEnshasy2023_ValidationoftheDetectionKitforPathogenicBacteriaSalmonella.pdf http://eprints.utm.my/107806/ http://dx.doi.org/10.1051/e3sconf/202340004009 |
| _version_ |
1814043527802257408 |
| score |
13.211869 |