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Validation of the detection kit for pathogenic bacteria salmonella typhi causes food poisoning with real time polymerase chain reaction.

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the...

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Main Authors: Maulana, Irvan, Nurjayadi, Muktiningsih, Ulfi Rahma Efrianti, Ulfi Rahma Efrianti, Azizah, Noer, Pramudiyasih, Nabilla Alya, Ratna Nur Kusumawati, Ratna Nur Kusumawati, Maharanianska Azzahra, Maharanianska Azzahra, Declan, Jefferson Lynford, Gladys Indira Putri, Gladys Indira Putri, Juliansyah, Dandi Akbar, Ismaya Krisdawati, Ismaya Krisdawati, Fera Kurniadewi, Fera Kurniadewi, Irma Ratna Kartika, Irma Ratna Kartika, Dalia Sukmawati, Dalia Sukmawati, Lidwina Nastassya, Lidwina Nastassya, Vira Saamia, Vira Saamia, Saputro, Dwi Anna Oktaviani, I Made Wiranatha, I Made Wiranatha, El-Enshasy, Hesham Ali
Format: Conference or Workshop Item
Language:English
Published: 2023
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/107806/1/HeshamAliElEnshasy2023_ValidationoftheDetectionKitforPathogenicBacteriaSalmonella.pdf
http://eprints.utm.my/107806/
http://dx.doi.org/10.1051/e3sconf/202340004009
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Summary:Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60oC and a standard DNA concentration of 50 ng/μL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to the standard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistent and reproducible data.

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