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A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode

Recently, conducting polymers have attracted much interest in the development of biosensor. It contain π- electron backbone responsible for its unusual electronic properties such as electrical conductivity, low energy optical transitions, low ionization potential and high electron affinity. Wh...

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Main Authors: Othman, Siti Amira, Radiman, Shahidan, Khoo, Kok Siong
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
T Technology (General)
Online Access:http://eprints.uthm.edu.my/7878/1/P9545_084e53bfecfe6657deeb5dee95a0ac82.pdf
http://eprints.uthm.edu.my/7878/
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spelling my.uthm.eprints.78782022-10-17T06:19:58Z http://eprints.uthm.edu.my/7878/ A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode Othman, Siti Amira Radiman, Shahidan Khoo, Kok Siong T Technology (General) Recently, conducting polymers have attracted much interest in the development of biosensor. It contain π- electron backbone responsible for its unusual electronic properties such as electrical conductivity, low energy optical transitions, low ionization potential and high electron affinity. When the Horseradish peroxidase (HRP) was immobilized to the conducting polymers, these polymers possesses the ability to bind oppositely charged complex entities in their neutral insulating state. Determination of Hydrogen peroxide (H2O2) and other organic peroxides is of practical importance in clinical, environmental and many other fields. This study intends to see the role and properties of PANI/HRP layer towards H2O2 by measuring its current. Langmuir- Blodgett technique was used to form the PANI monolayer and the HRP was deposited in PANI monolayer by using electrodeposition method. Results from U.V.- visible spectrum of PANI with and without HRP shows two sharp absorption peaks at 320 nm and 720 nm. PANI forms as nanoparticles was revealed by VPSEM. AFM shows the image in roughness before and after the HRP was deposited on PANI monolayer. The current and response of H2O2 towards PANI/HRP electrode increases demonstrating effective electrocatalytic reduction of H202. PANI/HRP electrode not only act as excellent materials for rapid electron transfer but also for the fabrication of efficient biosensors. 2010 Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/7878/1/P9545_084e53bfecfe6657deeb5dee95a0ac82.pdf Othman, Siti Amira and Radiman, Shahidan and Khoo, Kok Siong (2010) A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode. In: -.
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Othman, Siti Amira
Radiman, Shahidan
Khoo, Kok Siong
A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
description Recently, conducting polymers have attracted much interest in the development of biosensor. It contain π- electron backbone responsible for its unusual electronic properties such as electrical conductivity, low energy optical transitions, low ionization potential and high electron affinity. When the Horseradish peroxidase (HRP) was immobilized to the conducting polymers, these polymers possesses the ability to bind oppositely charged complex entities in their neutral insulating state. Determination of Hydrogen peroxide (H2O2) and other organic peroxides is of practical importance in clinical, environmental and many other fields. This study intends to see the role and properties of PANI/HRP layer towards H2O2 by measuring its current. Langmuir- Blodgett technique was used to form the PANI monolayer and the HRP was deposited in PANI monolayer by using electrodeposition method. Results from U.V.- visible spectrum of PANI with and without HRP shows two sharp absorption peaks at 320 nm and 720 nm. PANI forms as nanoparticles was revealed by VPSEM. AFM shows the image in roughness before and after the HRP was deposited on PANI monolayer. The current and response of H2O2 towards PANI/HRP electrode increases demonstrating effective electrocatalytic reduction of H202. PANI/HRP electrode not only act as excellent materials for rapid electron transfer but also for the fabrication of efficient biosensors.
format Conference or Workshop Item
author Othman, Siti Amira
Radiman, Shahidan
Khoo, Kok Siong
author_facet Othman, Siti Amira
Radiman, Shahidan
Khoo, Kok Siong
author_sort Othman, Siti Amira
title A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
title_short A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
title_full A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
title_fullStr A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
title_full_unstemmed A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
title_sort hydrogen peroxide biosensor based on nanoparticle pani/hrp electrode
publishDate 2010
url http://eprints.uthm.edu.my/7878/1/P9545_084e53bfecfe6657deeb5dee95a0ac82.pdf
http://eprints.uthm.edu.my/7878/
_version_ 1748182669242400768
score 13.211869

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