A hydrogen peroxide biosensor based on nanoparticle PANI/HRP electrode
Recently, conducting polymers have attracted much interest in the development of biosensor. It contain π- electron backbone responsible for its unusual electronic properties such as electrical conductivity, low energy optical transitions, low ionization potential and high electron affinity. Wh...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/7878/1/P9545_084e53bfecfe6657deeb5dee95a0ac82.pdf http://eprints.uthm.edu.my/7878/ |
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| Summary: | Recently, conducting polymers have attracted much interest in the development of
biosensor. It contain π- electron backbone responsible for its unusual electronic properties such
as electrical conductivity, low energy optical transitions, low ionization potential and high
electron affinity. When the Horseradish peroxidase (HRP) was immobilized to the conducting
polymers, these polymers possesses the ability to bind oppositely charged complex entities in
their neutral insulating state. Determination of Hydrogen peroxide (H2O2) and other organic
peroxides is of practical importance in clinical, environmental and many other fields. This study
intends to see the role and properties of PANI/HRP layer towards H2O2 by measuring its current.
Langmuir- Blodgett technique was used to form the PANI monolayer and the HRP was
deposited in PANI monolayer by using electrodeposition method. Results from U.V.- visible
spectrum of PANI with and without HRP shows two sharp absorption peaks at 320 nm and 720
nm. PANI forms as nanoparticles was revealed by VPSEM. AFM shows the image in
roughness before and after the HRP was deposited on PANI monolayer. The current and
response of H2O2 towards PANI/HRP electrode increases demonstrating effective
electrocatalytic reduction of H202. PANI/HRP electrode not only act as excellent materials for
rapid electron transfer but also for the fabrication of efficient biosensors. |
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