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Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)

Bambangan (Mangifera pajang) has been shown to exhibit anticancer activity. One of the major bioactive compounds present in the methanol extract of kernel of M. pajang is methyl 3,4,5-trihydroxybenzoate (methyl gallate). The present study was conducted to evaluate the cytotoxic activity of this comp...

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Main Authors: Rahim, A. Che, Abu Bakar, Mohd Fadzelly, Kassim, Kartinee, Stanslas, Johnson, Wan Mohamad Zain, Wan Nor I'zzah
Format: Article
Language:English
Published: Natural Anticancer Agents
Subjects:
T Technology (General)
Online Access:http://eprints.uthm.edu.my/5448/1/AJ%202018%20%28527%29.pdf
http://eprints.uthm.edu.my/5448/
https://doi.org/10.14233/ajchem.2018.21428
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spelling my.uthm.eprints.54482022-01-09T07:29:58Z http://eprints.uthm.edu.my/5448/ Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang) Rahim, A. Che Abu Bakar, Mohd Fadzelly Kassim, Kartinee Stanslas, Johnson Wan Mohamad Zain, Wan Nor I'zzah T Technology (General) Bambangan (Mangifera pajang) has been shown to exhibit anticancer activity. One of the major bioactive compounds present in the methanol extract of kernel of M. pajang is methyl 3,4,5-trihydroxybenzoate (methyl gallate). The present study was conducted to evaluate the cytotoxic activity of this compound against selected cancer cell lines such as hormone dependent breast cancer (MCF-7), nonhormone dependent breast cancer (MDA-MB-231), prostate cancer (PC-3), pancreatic cancer cell (CP-2) and colon cancer (HCT-116) cell lines. Methyl gallate was isolated from methanol extract by using column chromatography and the compound was further confirmed by using NMR, GC-MS analysis and comparison of spectral data of the isolated data with published report. The cytotoxicity of the compound was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at concentrationsranging from 0 to 100 µM. The results showed that the compound only induced cytotoxicity in MCF-7 and PC-3 cell lines with IC50 values of 54.7 ± 4.73 and 97.6 ± 4.04 µM, respectively. Furthermore, Capan 2, MDA-MB-231 and HCT-116 showed no inhibition towards the cell proliferation after the treatment with compound (IC50 values more than100 µM). Thus, the compound isolated from kernel of M. pajang exhibited selective cytotoxic activity against selected cancer cell lines. Natural Anticancer Agents Article PeerReviewed text en http://eprints.uthm.edu.my/5448/1/AJ%202018%20%28527%29.pdf Rahim, A. Che and Abu Bakar, Mohd Fadzelly and Kassim, Kartinee and Stanslas, Johnson and Wan Mohamad Zain, Wan Nor I'zzah Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang). Asian Journal of Chemistry, 30 (10). pp. 2273-2276. ISSN 0975-427X https://doi.org/10.14233/ajchem.2018.21428
institution Universiti Tun Hussein Onn Malaysia
building UTHM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Tun Hussein Onn Malaysia
content_source UTHM Institutional Repository
url_provider http://eprints.uthm.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Rahim, A. Che
Abu Bakar, Mohd Fadzelly
Kassim, Kartinee
Stanslas, Johnson
Wan Mohamad Zain, Wan Nor I'zzah
Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)
description Bambangan (Mangifera pajang) has been shown to exhibit anticancer activity. One of the major bioactive compounds present in the methanol extract of kernel of M. pajang is methyl 3,4,5-trihydroxybenzoate (methyl gallate). The present study was conducted to evaluate the cytotoxic activity of this compound against selected cancer cell lines such as hormone dependent breast cancer (MCF-7), nonhormone dependent breast cancer (MDA-MB-231), prostate cancer (PC-3), pancreatic cancer cell (CP-2) and colon cancer (HCT-116) cell lines. Methyl gallate was isolated from methanol extract by using column chromatography and the compound was further confirmed by using NMR, GC-MS analysis and comparison of spectral data of the isolated data with published report. The cytotoxicity of the compound was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at concentrationsranging from 0 to 100 µM. The results showed that the compound only induced cytotoxicity in MCF-7 and PC-3 cell lines with IC50 values of 54.7 ± 4.73 and 97.6 ± 4.04 µM, respectively. Furthermore, Capan 2, MDA-MB-231 and HCT-116 showed no inhibition towards the cell proliferation after the treatment with compound (IC50 values more than100 µM). Thus, the compound isolated from kernel of M. pajang exhibited selective cytotoxic activity against selected cancer cell lines.
format Article
author Rahim, A. Che
Abu Bakar, Mohd Fadzelly
Kassim, Kartinee
Stanslas, Johnson
Wan Mohamad Zain, Wan Nor I'zzah
author_facet Rahim, A. Che
Abu Bakar, Mohd Fadzelly
Kassim, Kartinee
Stanslas, Johnson
Wan Mohamad Zain, Wan Nor I'zzah
author_sort Rahim, A. Che
title Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)
title_short Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)
title_full Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)
title_fullStr Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)
title_full_unstemmed Selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from Kernel of Bambangan (Mangifera pajang)
title_sort selective cytotoxic activity of methyl-3,4,5-trihydroxybenzoate isolated from kernel of bambangan (mangifera pajang)
publisher Natural Anticancer Agents
url http://eprints.uthm.edu.my/5448/1/AJ%202018%20%28527%29.pdf
http://eprints.uthm.edu.my/5448/
https://doi.org/10.14233/ajchem.2018.21428
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score 13.211869

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