Potential antiproliferative properties of methanol and water extracts of pyrrosia piloselloides on human cervical carcinoma cell lines, HeLa
Cervical cancer is the third most commonly diagnosed cancer and the fourth leading cause of cancer death in female worldwide. Current estimates indicate that annually 2145 women are diagnosed with cervical cancer and 621 died from the disease. At present, the standard treatment for cervical carci...
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2016
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| Subjects: | |
| Online Access: | http://eprints.usm.my/61235/1/FASHIHAH%20BINTI%20ZAKARIA%20-%20e.pdf http://eprints.usm.my/61235/ |
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| Summary: | Cervical cancer is the third most commonly diagnosed cancer and the fourth leading
cause of cancer death in female worldwide. Current estimates indicate that annually
2145 women are diagnosed with cervical cancer and 621 died from the disease. At
present, the standard treatment for cervical carcinoma involves surgery, chemotherapy
and radiotherapy. Combined chemotherapy using Cisplatin appears to be effective as its
ability to initiate the apoptotic pathway. However, the use of Cisplatin is limited by its
toxicity and acquired chemoresistance throughout the treatment. In this study, we
investigated the potential antiproliferative effects of methanol and water extracts of
Pyrrosia piloselloides on HeLa cell lines. MTT assay was performed to determine the
IC5o concentration, whereby apoptosis analysis was done by flow cytometry to study the
ability of the plant extracts to induce apoptosis in the cell lines. P. piloselloides
methanol extracts (PPME) showed antiproliferative effect on HeLa cell lines with IC50
of 16.25pg/ml while P. piloselloides water extract (PPWE) showed no IC50 value. However, both extracts showed no significant effect in apoptosis assessment.
Identification of the chemical compounds exist in the extract was analysed by Gas Chromatography-Mass Spectrometry (GC-MS). Major compounds exist in PPME were 5-Hydroxymethylfurfural (23.13%), Allopurinol (8.66%) and 3,5-dihydroxy-6-methyl-
2,3-dihydropyran-4-one (7.41%) while PPWE were Sulfolan-3-ol (10.06%), Linoleic
acid (9.06%) and 0-Sitosterol acetate (7.98%). Further study needs to be done to investigate the reason and mechanism of anti-proliferative effect of methanol extract on
HeLa cell lines. |
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