Staff View: Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene

Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene

Although the introduction of antiretroviral (ARV) treatment has been associated with marked decreases in HIV-related morbidity and mortality, its effectiveness is limited by the emergence of drug resistance. This study was conducted to determine the presence of transmitted HIV drug resistance (TH...

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Main Author:	Ching, Hii Siew
Format:	Monograph
Language:	English
Published:	Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2013
Subjects:	RA643-645 Disease (Communicable and noninfectious) and public health RC Internal medicine
Online Access:	http://eprints.usm.my/57390/1/HII%20SIEW%20CHING-24%20pages.pdf http://eprints.usm.my/57390/
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spelling	my.usm.eprints.57390 http://eprints.usm.my/57390/ Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene Ching, Hii Siew RA643-645 Disease (Communicable and noninfectious) and public health RC Internal medicine Although the introduction of antiretroviral (ARV) treatment has been associated with marked decreases in HIV-related morbidity and mortality, its effectiveness is limited by the emergence of drug resistance. This study was conducted to determine the presence of transmitted HIV drug resistance (THIVDR) viruses and phylogenetic analysis of HIV-1 virus from naive ARV therapy patients from Kelantan, Malaysia based on RT gene. Our interest was on THIVDR as it makes ARV treatment more complicated and costly and there is limited information on transmitted HIVDR viruses were amplified and sequenced. After that, specific mutations of RT gene associated with drug resistance were analyzed using Standford HlVdb: Genotypic Resistance Interpretation Algorithm and HIV LANL ADRA: Antiviral Drug Resistance Analysis. From the result, mutations K101Q and K103N were identified in 3 and 1 of the studied HIV viruses respectively. These viruses are expected to have resistance to Nucleoside reverse-transcriptase inhibitors (NNRTI) Efavirenz drugs. Subsequently, these sequences were phylogenetically analyzed to determine the source and subtype of the viruses. Phylogenetic result showed that out of 10 HIV-1 viruses used in this study, 9 were of the CRF01AE subtype and only one was under CRF01AE/B subtype. This study also found that Kelantan HIV-1 viruses had high similarity (97-99%) to the Thailand HIV-1 viruses because these countries share a border with each other. In conclusion, transmitted HIVDRs were successfully identified in Kelantan, Malaysia. However, further study involving larger samples to determine the actual prevalence of THIVDR in Kelantan need to be carried out. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia 2013-05 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/57390/1/HII%20SIEW%20CHING-24%20pages.pdf Ching, Hii Siew (2013) Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene. Other. Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia. (Submitted)
institution	Universiti Sains Malaysia
building	Hamzah Sendut Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sains Malaysia
content_source	USM Institutional Repository
url_provider	http://eprints.usm.my/
language	English
topic	RA643-645 Disease (Communicable and noninfectious) and public health RC Internal medicine
spellingShingle	RA643-645 Disease (Communicable and noninfectious) and public health RC Internal medicine Ching, Hii Siew Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
description	Although the introduction of antiretroviral (ARV) treatment has been associated with marked decreases in HIV-related morbidity and mortality, its effectiveness is limited by the emergence of drug resistance. This study was conducted to determine the presence of transmitted HIV drug resistance (THIVDR) viruses and phylogenetic analysis of HIV-1 virus from naive ARV therapy patients from Kelantan, Malaysia based on RT gene. Our interest was on THIVDR as it makes ARV treatment more complicated and costly and there is limited information on transmitted HIVDR viruses were amplified and sequenced. After that, specific mutations of RT gene associated with drug resistance were analyzed using Standford HlVdb: Genotypic Resistance Interpretation Algorithm and HIV LANL ADRA: Antiviral Drug Resistance Analysis. From the result, mutations K101Q and K103N were identified in 3 and 1 of the studied HIV viruses respectively. These viruses are expected to have resistance to Nucleoside reverse-transcriptase inhibitors (NNRTI) Efavirenz drugs. Subsequently, these sequences were phylogenetically analyzed to determine the source and subtype of the viruses. Phylogenetic result showed that out of 10 HIV-1 viruses used in this study, 9 were of the CRF01AE subtype and only one was under CRF01AE/B subtype. This study also found that Kelantan HIV-1 viruses had high similarity (97-99%) to the Thailand HIV-1 viruses because these countries share a border with each other. In conclusion, transmitted HIVDRs were successfully identified in Kelantan, Malaysia. However, further study involving larger samples to determine the actual prevalence of THIVDR in Kelantan need to be carried out.
format	Monograph
author	Ching, Hii Siew
author_facet	Ching, Hii Siew
author_sort	Ching, Hii Siew
title	Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
title_short	Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
title_full	Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
title_fullStr	Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
title_full_unstemmed	Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
title_sort	transmitted drug resistance and phylogenetic analysis of hiv-1 virus from kelantan, malaysia based on reverse transcriptase (rt) gene
publisher	Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
publishDate	2013
url	http://eprints.usm.my/57390/1/HII%20SIEW%20CHING-24%20pages.pdf http://eprints.usm.my/57390/
_version_	1761617393466998784
score	13.211869

Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene

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