Transmitted drug resistance and phylogenetic analysis of HIV-1 virus from Kelantan, Malaysia based on Reverse Transcriptase (RT) gene
Although the introduction of antiretroviral (ARV) treatment has been associated with marked decreases in HIV-related morbidity and mortality, its effectiveness is limited by the emergence of drug resistance. This study was conducted to determine the presence of transmitted HIV drug resistance (TH...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
2013
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| Subjects: | |
| Online Access: | http://eprints.usm.my/57390/1/HII%20SIEW%20CHING-24%20pages.pdf http://eprints.usm.my/57390/ |
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| Summary: | Although the introduction of antiretroviral (ARV) treatment has been associated with
marked decreases in HIV-related morbidity and mortality, its effectiveness is limited
by the emergence of drug resistance. This study was conducted to determine the
presence of transmitted HIV drug resistance (THIVDR) viruses and phylogenetic
analysis of HIV-1 virus from naive ARV therapy patients from Kelantan, Malaysia
based on RT gene. Our interest was on THIVDR as it makes ARV treatment more
complicated and costly and there is limited information on transmitted HIVDR
viruses were amplified and sequenced. After that, specific mutations of RT gene
associated with drug resistance were analyzed using Standford HlVdb: Genotypic
Resistance Interpretation Algorithm and HIV LANL ADRA: Antiviral Drug
Resistance Analysis. From the result, mutations K101Q and K103N were identified
in 3 and 1 of the studied HIV viruses respectively. These viruses are expected to
have resistance to Nucleoside reverse-transcriptase inhibitors (NNRTI) Efavirenz
drugs. Subsequently, these sequences were phylogenetically analyzed to determine
the source and subtype of the viruses. Phylogenetic result showed that out of 10
HIV-1 viruses used in this study, 9 were of the CRF01AE subtype and only one was
under CRF01AE/B subtype. This study also found that Kelantan HIV-1 viruses had
high similarity (97-99%) to the Thailand HIV-1 viruses because these countries share
a border with each other. In conclusion, transmitted HIVDRs were successfully
identified in Kelantan, Malaysia. However, further study involving larger samples to
determine the actual prevalence of THIVDR in Kelantan need to be carried out. |
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