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Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast

Cbitosan has been used in several studies as wound healing accelerator. This study was designed to determine the cytotoxicity of cbitosan on primary cultured human fibroblasts. Human skins were obtained from surgical specimen of consented donor and primary cultured fibroblasts were established. T...

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Main Author: Nurul Firdaus, Abdul Hadi
Format: Monograph
Language:English
Published: Universiti Sains Malaysia 2006
Subjects:
R Medicine
Online Access:http://eprints.usm.my/48587/1/Nurul%20Firdaus%20Binti%20Abdul%20Hadi%20-%2024%20pages.pdf
http://eprints.usm.my/48587/
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spelling my.usm.eprints.48587 http://eprints.usm.my/48587/ Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast Nurul Firdaus, Abdul Hadi R Medicine Cbitosan has been used in several studies as wound healing accelerator. This study was designed to determine the cytotoxicity of cbitosan on primary cultured human fibroblasts. Human skins were obtained from surgical specimen of consented donor and primary cultured fibroblasts were established. The fibroblasts were incubated in the absence or presence of increasing concentrations of Oligo Chito 1% and Oligo Chito 5% for 24, 48 and 72 hours. Subsequently, MIT reagent was added and 4 hours later the purple crystals produced were dissolved in DMSO. Then, cells viability was read using ELISA plate reader. Cells viability was used as a marker for toxicity. The results showed that cell viability was dependent on concentration. The cell viability decreased when high concentrations of chitosan were used. Prolonged incubation period also caused decreased cell viability. Analysis of chitosan effects on cell culture is useful as a screening tool for their potential in vivo activity as wound healing agents. Universiti Sains Malaysia 2006-03 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/48587/1/Nurul%20Firdaus%20Binti%20Abdul%20Hadi%20-%2024%20pages.pdf Nurul Firdaus, Abdul Hadi (2006) Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast. Other. Universiti Sains Malaysia. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R Medicine
spellingShingle R Medicine
Nurul Firdaus, Abdul Hadi
Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
description Cbitosan has been used in several studies as wound healing accelerator. This study was designed to determine the cytotoxicity of cbitosan on primary cultured human fibroblasts. Human skins were obtained from surgical specimen of consented donor and primary cultured fibroblasts were established. The fibroblasts were incubated in the absence or presence of increasing concentrations of Oligo Chito 1% and Oligo Chito 5% for 24, 48 and 72 hours. Subsequently, MIT reagent was added and 4 hours later the purple crystals produced were dissolved in DMSO. Then, cells viability was read using ELISA plate reader. Cells viability was used as a marker for toxicity. The results showed that cell viability was dependent on concentration. The cell viability decreased when high concentrations of chitosan were used. Prolonged incubation period also caused decreased cell viability. Analysis of chitosan effects on cell culture is useful as a screening tool for their potential in vivo activity as wound healing agents.
format Monograph
author Nurul Firdaus, Abdul Hadi
author_facet Nurul Firdaus, Abdul Hadi
author_sort Nurul Firdaus, Abdul Hadi
title Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
title_short Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
title_full Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
title_fullStr Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
title_full_unstemmed Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
title_sort cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
publisher Universiti Sains Malaysia
publishDate 2006
url http://eprints.usm.my/48587/1/Nurul%20Firdaus%20Binti%20Abdul%20Hadi%20-%2024%20pages.pdf
http://eprints.usm.my/48587/
_version_ 1695531540468989952
score 13.211869

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