Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast
Cbitosan has been used in several studies as wound healing accelerator. This study was designed to determine the cytotoxicity of cbitosan on primary cultured human fibroblasts. Human skins were obtained from surgical specimen of consented donor and primary cultured fibroblasts were established. T...
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| Main Author: | |
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2006
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| Online Access: | http://eprints.usm.my/48587/1/Nurul%20Firdaus%20Binti%20Abdul%20Hadi%20-%2024%20pages.pdf http://eprints.usm.my/48587/ |
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| Summary: | Cbitosan has been used in several studies as wound healing accelerator. This study
was designed to determine the cytotoxicity of cbitosan on primary cultured human
fibroblasts. Human skins were obtained from surgical specimen of consented donor and
primary cultured fibroblasts were established. The fibroblasts were incubated in the
absence or presence of increasing concentrations of Oligo Chito 1% and Oligo Chito 5%
for 24, 48 and 72 hours. Subsequently, MIT reagent was added and 4 hours later the
purple crystals produced were dissolved in DMSO. Then, cells viability was read using
ELISA plate reader. Cells viability was used as a marker for toxicity. The results showed
that cell viability was dependent on concentration. The cell viability decreased when high
concentrations of chitosan were used. Prolonged incubation period also caused decreased
cell viability. Analysis of chitosan effects on cell culture is useful as a screening tool for
their potential in vivo activity as wound healing agents. |
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