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Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease

Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes...

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Main Authors: Rashid, Faridah Abdul, Mustapha, Mohd Rafi
Format: Conference or Workshop Item
Language:English
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RC666-701 Diseases of the circulatory (Cardiovascular) system
Online Access:http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf
http://eprints.usm.my/42635/
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spelling my.usm.eprints.42635 http://eprints.usm.my/42635/ Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease Rashid, Faridah Abdul Mustapha, Mohd Rafi RC666-701 Diseases of the circulatory (Cardiovascular) system Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes, DNA were extracted from huffy coat amplified by PCR, restricted by endonuclease, electrophoresed on polyacrylamide gel and visualised by ethidium bromide staining. The genotypes obtained were: E-3/3(64%), E-4/3(27%), E-3/2(5%), and E-4/2(4%), and gene frequencies were: £2(0.04), e3(0.80), £4(0.16). The E-3/3 genotype contributed to hypercholesterolemia, low HDLC, high LDLC high ApoB. The E-4/2 genotype gave the opposite effect of E-3/3. The E-4/3 genotype exhibited hypertriglyceridemia, low LDLC and low ApoB. The E-3/2 genotype manifested normotriglyceridemia, high HDLC, low ApoA-I and low AlB ratio. We conclude that the E-3/3 genotype had the highest risk for premature heart disease. Those with the other genotypes (E-4/3, E-4/2 and E-3/2) had lower risk of premature heart disease. Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf Rashid, Faridah Abdul and Mustapha, Mohd Rafi Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease. In: Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease, 8-9 June 1998, USM Kubang Kerian. (Submitted)
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic RC666-701 Diseases of the circulatory (Cardiovascular) system
spellingShingle RC666-701 Diseases of the circulatory (Cardiovascular) system
Rashid, Faridah Abdul
Mustapha, Mohd Rafi
Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
description Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes, DNA were extracted from huffy coat amplified by PCR, restricted by endonuclease, electrophoresed on polyacrylamide gel and visualised by ethidium bromide staining. The genotypes obtained were: E-3/3(64%), E-4/3(27%), E-3/2(5%), and E-4/2(4%), and gene frequencies were: £2(0.04), e3(0.80), £4(0.16). The E-3/3 genotype contributed to hypercholesterolemia, low HDLC, high LDLC high ApoB. The E-4/2 genotype gave the opposite effect of E-3/3. The E-4/3 genotype exhibited hypertriglyceridemia, low LDLC and low ApoB. The E-3/2 genotype manifested normotriglyceridemia, high HDLC, low ApoA-I and low AlB ratio. We conclude that the E-3/3 genotype had the highest risk for premature heart disease. Those with the other genotypes (E-4/3, E-4/2 and E-3/2) had lower risk of premature heart disease.
format Conference or Workshop Item
author Rashid, Faridah Abdul
Mustapha, Mohd Rafi
author_facet Rashid, Faridah Abdul
Mustapha, Mohd Rafi
author_sort Rashid, Faridah Abdul
title Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
title_short Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
title_full Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
title_fullStr Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
title_full_unstemmed Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease
title_sort effect of apolipoprotein e genotype variation on the risk of premature ischaemic heart disease
url http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf
http://eprints.usm.my/42635/
_version_ 1662755731658506240
score 13.244745

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