Effect of apolipoprotein E genotype variation on the risk of premature ischaemic heart disease

Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes...

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Bibliographic Details
Main Authors: Rashid, Faridah Abdul, Mustapha, Mohd Rafi
Format: Conference or Workshop Item
Language:English
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Online Access:http://eprints.usm.my/42635/1/GP...Kesan_Variasi_Genotip_Apolipoprotein_E_Ke_Atas_Risiko_Penyakit_Premature_Ischaemic_Heart_Disease...OCR....pdf
http://eprints.usm.my/42635/
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Summary:Apolipoprotein E is expressed by 3 allelic genes, £2, £3 and £4, on Chromosome 19. ApoE isoforms are distinguished by residues at positions 112 and 158. We analysed blood from 83 first year medical students for: Total cholesterol and triglycerides, HDLC, LDLC, ApoA-1 and B levels. For ApoE genotypes, DNA were extracted from huffy coat amplified by PCR, restricted by endonuclease, electrophoresed on polyacrylamide gel and visualised by ethidium bromide staining. The genotypes obtained were: E-3/3(64%), E-4/3(27%), E-3/2(5%), and E-4/2(4%), and gene frequencies were: £2(0.04), e3(0.80), £4(0.16). The E-3/3 genotype contributed to hypercholesterolemia, low HDLC, high LDLC high ApoB. The E-4/2 genotype gave the opposite effect of E-3/3. The E-4/3 genotype exhibited hypertriglyceridemia, low LDLC and low ApoB. The E-3/2 genotype manifested normotriglyceridemia, high HDLC, low ApoA-I and low AlB ratio. We conclude that the E-3/3 genotype had the highest risk for premature heart disease. Those with the other genotypes (E-4/3, E-4/2 and E-3/2) had lower risk of premature heart disease.