Staff View: Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation

Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation

Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain...

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Main Authors:	Abdelwahab, Siddig Ibrahim, Hassan, Loiy Elsir Ahmed, Abdul Majid, Amin M. S., Ahmed Yagi, Sakina M., Mohan, Syam, Mohamed Elhassan Taha, Manal, Ahmad, Syahida, Shiau Chuen, Cheah, Mohd Fauzi, Putri Narrima, Rais, Mohd Mustafa, Syam, Suvitha, Abdulkarim Moharam, Bushra, A. Hadi, A. Hamid
Format:	Article
Language:	English
Published:	Hindawi Publishing Corporation 2012
Subjects:	RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/38597/1/Cucurbitacin_L_2-O-%CE%B2-Glucoside_Demonstrates_Apoptogenesis_in_Colon_Adenocarcinoma_Cells.pdf http://eprints.usm.my/38597/ https://doi.org/10.1155/2012/490136
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id	my.usm.eprints.38597
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spelling	my.usm.eprints.38597 http://eprints.usm.my/38597/ Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results revealed that CLG was cytotoxic to HT-29. CLG increased significantly (P < 0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and realtime qPCR, respectively. The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide (NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it. This compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using the MTT assay. Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays demonstrate the antioxidant properties of CLG. The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://eprints.usm.my/38597/1/Cucurbitacin_L_2-O-%CE%B2-Glucoside_Demonstrates_Apoptogenesis_in_Colon_Adenocarcinoma_Cells.pdf Abdelwahab, Siddig Ibrahim and Hassan, Loiy Elsir Ahmed and Abdul Majid, Amin M. S. and Ahmed Yagi, Sakina M. and Mohan, Syam and Mohamed Elhassan Taha, Manal and Ahmad, Syahida and Shiau Chuen, Cheah and Mohd Fauzi, Putri Narrima and Rais, Mohd Mustafa and Syam, Suvitha and Abdulkarim Moharam, Bushra and A. Hadi, A. Hamid (2012) Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation. Evidence-Based Complementary and Alternative Medicine, 2012 (490136). pp. 1-8. ISSN 1741-427X https://doi.org/10.1155/2012/490136
institution	Universiti Sains Malaysia
building	Hamzah Sendut Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sains Malaysia
content_source	USM Institutional Repository
url_provider	http://eprints.usm.my/
language	English
topic	RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica
spellingShingle	RM Therapeutics. Pharmacology RS1-441 Pharmacy and materia medica Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
description	Emerging evidence suggests that reactive oxygen (ROS) and nitrogen (RNS) species can contribute to diverse signalling pathways of inflammatory and tumour cells. Cucurbitacins are a group of highly oxygenated triterpenes. Many plants used in folk medicine to treat cancer have been found to contain cucurbitacins displaying potentially important anti-inflammatory actions. The current study was designed to investigate the anti-ROS and -RNS effects of cucurbitacin L 2-O-β-glucoside (CLG) and the role of these signaling factors in the apoptogenic effects of CLG on human colon cancer cells (HT-29). This natural cucurbitacin was isolated purely from Citrullus lanatus var. citroides (Cucurbitaceae). The results revealed that CLG was cytotoxic to HT-29. CLG increased significantly (P < 0.05) RNA and protein levels of caspase-3 in HT-29 cells when verified using a colorimetric assay and realtime qPCR, respectively. The results showed that lipopolysaccharide/interferon-gamma (LPS/INF-γ) increased nitrous oxide (NO) production inR AW264.7macrophages, whereas N(G)-nitro-L-argininemethyl ester (L-NAME) and CLG curtailed it. This compound did not reveal any cytotoxicity on RAW264.7 macrophages and human normal liver cells (WRL-68) when tested using the MTT assay. Findings of ferric reducing antioxidant power (FRAP) and oxygen radical absorption capacity (ORAC) assays demonstrate the antioxidant properties of CLG. The apoptogenic property of CLG on HT-29 cells is thus related to inhibition of reactive nitrogen and oxygen reactive species and the triggering of caspase-3-regulated apoptosis.
format	Article
author	Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid
author_facet	Abdelwahab, Siddig Ibrahim Hassan, Loiy Elsir Ahmed Abdul Majid, Amin M. S. Ahmed Yagi, Sakina M. Mohan, Syam Mohamed Elhassan Taha, Manal Ahmad, Syahida Shiau Chuen, Cheah Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa Syam, Suvitha Abdulkarim Moharam, Bushra A. Hadi, A. Hamid
author_sort	Abdelwahab, Siddig Ibrahim
title	Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
title_short	Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
title_full	Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
title_fullStr	Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
title_full_unstemmed	Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation
title_sort	cucurbitacin l 2-o-β-glucoside demonstrates apoptogenesis in colon adenocarcinoma cells (ht-29): involvement of reactive oxygen and nitrogen species regulation
publisher	Hindawi Publishing Corporation
publishDate	2012
url	http://eprints.usm.my/38597/1/Cucurbitacin_L_2-O-%CE%B2-Glucoside_Demonstrates_Apoptogenesis_in_Colon_Adenocarcinoma_Cells.pdf http://eprints.usm.my/38597/ https://doi.org/10.1155/2012/490136
_version_	1643709404590112768
score	13.211869

Cucurbitacin L 2-O-β-Glucoside Demonstrates Apoptogenesis in Colon Adenocarcinoma Cells (HT-29): Involvement of Reactive Oxygen and Nitrogen Species Regulation

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