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Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla

A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPileamicrophylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPHfree radical scavenging, also total...

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Main Authors: Chahardehi, Amir Modarresi, Ibrahim, Darah, Sulaiman, Shaida Fariza
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2010
Subjects:
QH1 Natural history (General - Including nature conservation, geographical distribution)
Online Access:http://eprints.usm.my/38339/1/Antioxidant%2C_Antimicrobial_Activity_and_Toxicity.pdf
http://eprints.usm.my/38339/
http://doi.org/10.1155/2010/826830
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spelling my.usm.eprints.38339 http://eprints.usm.my/38339/ Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla Chahardehi, Amir Modarresi Ibrahim, Darah Sulaiman, Shaida Fariza QH1 Natural history (General - Including nature conservation, geographical distribution) A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPileamicrophylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPHfree radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps.Methanol extract of method I (ME I) showed the highest antioxidant activity at 69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at 72.10±0.71 and chloroformextract of method II (CE II) showed the highest total flavonoid contents at 60.14 ± 0.33. The antimicrobial activity of Pilea microphylla extract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). The Pilea microphylla extract showed antibacterial activity against some Gramnegative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry. Hindawi Publishing Corporation 2010 Article PeerReviewed application/pdf en http://eprints.usm.my/38339/1/Antioxidant%2C_Antimicrobial_Activity_and_Toxicity.pdf Chahardehi, Amir Modarresi and Ibrahim, Darah and Sulaiman, Shaida Fariza (2010) Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla. International Journal of Microbiology, 2010 (826830). pp. 1-6. ISSN 1687-918X http://doi.org/10.1155/2010/826830
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic QH1 Natural history (General - Including nature conservation, geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation, geographical distribution)
Chahardehi, Amir Modarresi
Ibrahim, Darah
Sulaiman, Shaida Fariza
Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
description A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPileamicrophylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPHfree radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps.Methanol extract of method I (ME I) showed the highest antioxidant activity at 69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at 72.10±0.71 and chloroformextract of method II (CE II) showed the highest total flavonoid contents at 60.14 ± 0.33. The antimicrobial activity of Pilea microphylla extract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). The Pilea microphylla extract showed antibacterial activity against some Gramnegative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.
format Article
author Chahardehi, Amir Modarresi
Ibrahim, Darah
Sulaiman, Shaida Fariza
author_facet Chahardehi, Amir Modarresi
Ibrahim, Darah
Sulaiman, Shaida Fariza
author_sort Chahardehi, Amir Modarresi
title Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
title_short Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
title_full Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
title_fullStr Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
title_full_unstemmed Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
title_sort antioxidant, antimicrobial activity and toxicity test of pileamicrophylla
publisher Hindawi Publishing Corporation
publishDate 2010
url http://eprints.usm.my/38339/1/Antioxidant%2C_Antimicrobial_Activity_and_Toxicity.pdf
http://eprints.usm.my/38339/
http://doi.org/10.1155/2010/826830
_version_ 1643709328477126656
score 13.211869

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