Antioxidant, Antimicrobial Activity and Toxicity Test of Pileamicrophylla
A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPileamicrophylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPHfree radical scavenging, also total...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Hindawi Publishing Corporation
2010
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Subjects: | |
Online Access: | http://eprints.usm.my/38339/1/Antioxidant%2C_Antimicrobial_Activity_and_Toxicity.pdf http://eprints.usm.my/38339/ http://doi.org/10.1155/2010/826830 |
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Summary: | A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial
activities fromPileamicrophylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPHfree
radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using
brine shrimps.Methanol extract of method I (ME I) showed the highest antioxidant activity at 69.51±1.03. Chloroform extract of
method I (CE I) showed the highest total phenolic contents at 72.10±0.71 and chloroformextract of method II (CE II) showed the
highest total flavonoid contents at 60.14 ± 0.33. The antimicrobial activity of Pilea microphylla extract was tested in vitro by using
disc diffusion method and minimum inhibitory concentration (MIC). The Pilea microphylla extract showed antibacterial activity
against some Gramnegative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract
of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as
antimicrobial and antioxidative agents in food industry. |
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