The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of Candida albicans Biofilms
Background: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric substances, morphogenetic swi...
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Ahvaz Jundishapur University of Medical Sciences
2016
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my.usm.eprints.37566 http://eprints.usm.my/37566/ The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of Candida albicans Biofilms Ishola, Oluwaseun Ayodeji Seng, Yeat Ting Tabana, Yasser M. Ahmed Adam, Mowaffaq Adam Yunus, Muhammad Amir Mohamed, Rafeezul Thian, Leslie Lung Than Sandai, Doblin QR1-502 Microbiology Background: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric substances, morphogenetic switching, and metabolic flexibility. Objectives: This study evaluated the roles of metabolic processes (in particular the glyoxylate cycle) on biofilm formation, antifungal drug resistance, morphology, and cell wall components. Methods: Growth, adhesion, biofilm formation, and cell wall carbohydrate composition were quantified for isogenic Candida albicans ICL1/ICL1, ICL1/icl1, and icl1/icl1 strains. The morphology and topography of these strains were compared by light microscopy and scanning electron microscopy. FKS1 (glucan synthase), ERG11 (14-demethylase), and CDR2 (efflux pump) mRNA levels were quantified using qRT-PCR. Results: The ICL1/icl1 and icl1/icl1 strains formed similar biofilms and exhibited analogous drug-tolerance levels to the control ICL1/ICL1 strains. Furthermore, the drug sequestration ability of -1, 3-glucan, a major carbohydrate component of the extracellular matrix, was not impaired. However, the inactivation of ICL1 did impair morphogenesis. ICL1 deletion also had a considerable effect on the expression of the FKS1, ERG11, and CDR2 genes. FKS1 and ERG11 were upregulated in ICL1/icl1 and icl1/icl1 cells throughout the biofilm developmental stages, and CDR2 was upregulated at the early phase. However, their expression was downregulated compared to the control ICL1/ICL1 strain. Conclusions: We conclude that the glyoxylate cycle is not a specific determinant of biofilm drug resistance. Ahvaz Jundishapur University of Medical Sciences 2016 Article PeerReviewed application/pdf en cc_by http://eprints.usm.my/37566/1/The_Role_of_Isocitrate_Lyase_ICL1_in_the_Metabolic.pdf Ishola, Oluwaseun Ayodeji and Seng, Yeat Ting and Tabana, Yasser M. and Ahmed Adam, Mowaffaq Adam and Yunus, Muhammad Amir and Mohamed, Rafeezul and Thian, Leslie Lung Than and Sandai, Doblin (2016) The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of Candida albicans Biofilms. Jundishapur Journal of Microbiology, 9 (9). pp. 1-14. ISSN 2008-3645 http://jjmicrobiol.com/en/articles/56959.html |
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QR1-502 Microbiology |
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QR1-502 Microbiology Ishola, Oluwaseun Ayodeji Seng, Yeat Ting Tabana, Yasser M. Ahmed Adam, Mowaffaq Adam Yunus, Muhammad Amir Mohamed, Rafeezul Thian, Leslie Lung Than Sandai, Doblin The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of Candida albicans Biofilms |
| description |
Background: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance
to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric
substances, morphogenetic switching, and metabolic flexibility.
Objectives: This study evaluated the roles of metabolic processes (in particular the glyoxylate cycle) on biofilm formation, antifungal
drug resistance, morphology, and cell wall components.
Methods: Growth, adhesion, biofilm formation, and cell wall carbohydrate composition were quantified for isogenic Candida albicans
ICL1/ICL1, ICL1/icl1, and icl1/icl1 strains. The morphology and topography of these strains were compared by light microscopy and
scanning electron microscopy. FKS1 (glucan synthase), ERG11 (14-demethylase), and CDR2 (efflux pump) mRNA levels were quantified
using qRT-PCR.
Results: The ICL1/icl1 and icl1/icl1 strains formed similar biofilms and exhibited analogous drug-tolerance levels to the control
ICL1/ICL1 strains. Furthermore, the drug sequestration ability of -1, 3-glucan, a major carbohydrate component of the extracellular
matrix, was not impaired. However, the inactivation of ICL1 did impair morphogenesis. ICL1 deletion also had a considerable
effect on the expression of the FKS1, ERG11, and CDR2 genes. FKS1 and ERG11 were upregulated in ICL1/icl1 and icl1/icl1 cells throughout
the biofilm developmental stages, and CDR2 was upregulated at the early phase. However, their expression was downregulated
compared to the control ICL1/ICL1 strain.
Conclusions: We conclude that the glyoxylate cycle is not a specific determinant of biofilm drug resistance. |
| format |
Article |
| author |
Ishola, Oluwaseun Ayodeji Seng, Yeat Ting Tabana, Yasser M. Ahmed Adam, Mowaffaq Adam Yunus, Muhammad Amir Mohamed, Rafeezul Thian, Leslie Lung Than Sandai, Doblin |
| author_facet |
Ishola, Oluwaseun Ayodeji Seng, Yeat Ting Tabana, Yasser M. Ahmed Adam, Mowaffaq Adam Yunus, Muhammad Amir Mohamed, Rafeezul Thian, Leslie Lung Than Sandai, Doblin |
| author_sort |
Ishola, Oluwaseun Ayodeji |
| title |
The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of
Candida albicans Biofilms |
| title_short |
The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of
Candida albicans Biofilms |
| title_full |
The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of
Candida albicans Biofilms |
| title_fullStr |
The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of
Candida albicans Biofilms |
| title_full_unstemmed |
The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of
Candida albicans Biofilms |
| title_sort |
role of isocitrate lyase (icl1) in the metabolic adaptation of
candida albicans biofilms |
| publisher |
Ahvaz Jundishapur University of Medical Sciences |
| publishDate |
2016 |
| url |
http://eprints.usm.my/37566/1/The_Role_of_Isocitrate_Lyase_ICL1_in_the_Metabolic.pdf http://eprints.usm.my/37566/ http://jjmicrobiol.com/en/articles/56959.html |
| _version_ |
1643709105608589312 |
| score |
13.251813 |