The Role of Isocitrate Lyase (ICL1) in the Metabolic Adaptation of Candida albicans Biofilms
Background: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric substances, morphogenetic swi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ahvaz Jundishapur University of Medical Sciences
2016
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/37566/1/The_Role_of_Isocitrate_Lyase_ICL1_in_the_Metabolic.pdf http://eprints.usm.my/37566/ http://jjmicrobiol.com/en/articles/56959.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Background: A major characteristic of Candida biofilm cells that differentiates them from free-floating cells is their high tolerance
to antifungal drugs. This high resistance is attributed to particular biofilm properties, including the accumulation of extrapolymeric
substances, morphogenetic switching, and metabolic flexibility.
Objectives: This study evaluated the roles of metabolic processes (in particular the glyoxylate cycle) on biofilm formation, antifungal
drug resistance, morphology, and cell wall components.
Methods: Growth, adhesion, biofilm formation, and cell wall carbohydrate composition were quantified for isogenic Candida albicans
ICL1/ICL1, ICL1/icl1, and icl1/icl1 strains. The morphology and topography of these strains were compared by light microscopy and
scanning electron microscopy. FKS1 (glucan synthase), ERG11 (14-demethylase), and CDR2 (efflux pump) mRNA levels were quantified
using qRT-PCR.
Results: The ICL1/icl1 and icl1/icl1 strains formed similar biofilms and exhibited analogous drug-tolerance levels to the control
ICL1/ICL1 strains. Furthermore, the drug sequestration ability of -1, 3-glucan, a major carbohydrate component of the extracellular
matrix, was not impaired. However, the inactivation of ICL1 did impair morphogenesis. ICL1 deletion also had a considerable
effect on the expression of the FKS1, ERG11, and CDR2 genes. FKS1 and ERG11 were upregulated in ICL1/icl1 and icl1/icl1 cells throughout
the biofilm developmental stages, and CDR2 was upregulated at the early phase. However, their expression was downregulated
compared to the control ICL1/ICL1 strain.
Conclusions: We conclude that the glyoxylate cycle is not a specific determinant of biofilm drug resistance. |
|---|