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In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique

Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL...

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Main Authors: Wai, Yean Leong, Chin, Fhong Soon, Soon, Chuan Wong, Kian, Sek Tee, Sok, Ching Cheong, Siew, Hua Gan, Youseffi, Mansour
Format: Article
Language:English
Published: MDPI 2017
Subjects:
R5-130.5 General works
Online Access:http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf
http://eprints.usm.my/36986/
http://www.mdpi.com/2306-5354/4/2/43
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spelling my.usm.eprints.36986 http://eprints.usm.my/36986/ In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique Wai, Yean Leong Chin, Fhong Soon Soon, Chuan Wong Kian, Sek Tee Sok, Ching Cheong Siew, Hua Gan Youseffi, Mansour R5-130.5 General works Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies. MDPI 2017 Article PeerReviewed application/pdf en http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf Wai, Yean Leong and Chin, Fhong Soon and Soon, Chuan Wong and Kian, Sek Tee and Sok, Ching Cheong and Siew, Hua Gan and Youseffi, Mansour (2017) In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique. Bioengineering, 4 (43). pp. 1-14. ISSN 2306-5354 http://www.mdpi.com/2306-5354/4/2/43
institution Universiti Sains Malaysia
building Hamzah Sendut Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sains Malaysia
content_source USM Institutional Repository
url_provider http://eprints.usm.my/
language English
topic R5-130.5 General works
spellingShingle R5-130.5 General works
Wai, Yean Leong
Chin, Fhong Soon
Soon, Chuan Wong
Kian, Sek Tee
Sok, Ching Cheong
Siew, Hua Gan
Youseffi, Mansour
In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
description Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.
format Article
author Wai, Yean Leong
Chin, Fhong Soon
Soon, Chuan Wong
Kian, Sek Tee
Sok, Ching Cheong
Siew, Hua Gan
Youseffi, Mansour
author_facet Wai, Yean Leong
Chin, Fhong Soon
Soon, Chuan Wong
Kian, Sek Tee
Sok, Ching Cheong
Siew, Hua Gan
Youseffi, Mansour
author_sort Wai, Yean Leong
title In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
title_short In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
title_full In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
title_fullStr In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
title_full_unstemmed In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
title_sort in vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
publisher MDPI
publishDate 2017
url http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf
http://eprints.usm.my/36986/
http://www.mdpi.com/2306-5354/4/2/43
_version_ 1643708940381323264
score 13.211869

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