In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique
Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI
2017
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| Subjects: | |
| Online Access: | http://eprints.usm.my/36986/1/%28In_Vitro_Growth_of_Human_%29__bioengineering-04-00043.pdf http://eprints.usm.my/36986/ http://www.mdpi.com/2306-5354/4/2/43 |
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| Summary: | Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue
transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model
for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line
(ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min
and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate
microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m.
Both cell lines were successfully grown into microtissues in the microcapsules of alginate within
16 days of culture. The microtissues were characterized by using a live/dead cell viability assay,
field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating
experiments. The microtissues of both cell types were viable after being extracted from the alginate
membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated
differences in both nucleus size and morphology. The microtissues with re-associated cells in
spheroids are potentially useful as a cell model for pharmacological studies. |
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