Cover Image

Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method

The usage of non-halal plasma transglutaminase to improve the gelling properties of surimi is prohibited for Muslim consumers. The objective of this study is to detect non-halal plasma transglutaminase in surimi products. A total of 12 samples were tested using DEAE, Unosphere Q and BioScale Macropr...

Full description

Saved in:
Bibliographic Details
Main Authors: A.R., Alina, M.A., Nur Illiyin, Y., Salmah, A., Siti Mashitoh, A.K., Imtinan, J., Juriani
Format: Article
Language:en_US
Published: 2015
Subjects:
Monoclonal antibody
Sandwich ELISA
Surimi
Textural
Online Access:http://ddms.usim.edu.my/handle/123456789/8774
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.usim-8774
record_format dspace
spelling my.usim-87742017-02-23T07:13:29Z Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method A.R., Alina M.A., Nur Illiyin Y., Salmah A., Siti Mashitoh A.K., Imtinan J., Juriani Monoclonal antibody Sandwich ELISA Surimi Textural The usage of non-halal plasma transglutaminase to improve the gelling properties of surimi is prohibited for Muslim consumers. The objective of this study is to detect non-halal plasma transglutaminase in surimi products. A total of 12 samples were tested using DEAE, Unosphere Q and BioScale Macroprep High Q columns and further confirmed by Sandwich ELISA method. Three different monoclonal antibody (MAbs) species which were bovine, chicken and porcine were used to observe the reaction against the samples. The reactivity of the antibody against the antigen was defined in a certain range of cutoff value that is very strong, strong, moderate, weak and negative. By using the MAbs of the different species, the result showed S1, S2 and S3 did not contain transglutaminase from bovine while the other samples did. Six samples which were S1, S2, S3, S8, S11 and S12 selected in the ELISA procedure had a very strong reaction with transglutaminase from porcine species. For MAbs of chicken species, S12 has a weak reactivity while other samples showed very strong and strong reaction of transglutaminase. The sandwich ELISA can be a useful method to detect the presence of transglutaminase in surimi-based products, which is derived from blood of different species of mammalian animals. Further study should be done to optimize the specificity of antibody used in the confirmation of TGase in surimi. © IDOSI Publications, 2012. 2015-07-13T05:01:23Z 2015-07-13T05:01:23Z 2012 Article 18184952 http://ddms.usim.edu.my/handle/123456789/8774 en_US
institution Universiti Sains Islam Malaysia
building USIM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universit Sains Islam i Malaysia
content_source USIM Institutional Repository
url_provider http://ddms.usim.edu.my/
language en_US
topic Monoclonal antibody
Sandwich ELISA
Surimi
Textural
spellingShingle Monoclonal antibody
Sandwich ELISA
Surimi
Textural
A.R., Alina
M.A., Nur Illiyin
Y., Salmah
A., Siti Mashitoh
A.K., Imtinan
J., Juriani
Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method
description The usage of non-halal plasma transglutaminase to improve the gelling properties of surimi is prohibited for Muslim consumers. The objective of this study is to detect non-halal plasma transglutaminase in surimi products. A total of 12 samples were tested using DEAE, Unosphere Q and BioScale Macroprep High Q columns and further confirmed by Sandwich ELISA method. Three different monoclonal antibody (MAbs) species which were bovine, chicken and porcine were used to observe the reaction against the samples. The reactivity of the antibody against the antigen was defined in a certain range of cutoff value that is very strong, strong, moderate, weak and negative. By using the MAbs of the different species, the result showed S1, S2 and S3 did not contain transglutaminase from bovine while the other samples did. Six samples which were S1, S2, S3, S8, S11 and S12 selected in the ELISA procedure had a very strong reaction with transglutaminase from porcine species. For MAbs of chicken species, S12 has a weak reactivity while other samples showed very strong and strong reaction of transglutaminase. The sandwich ELISA can be a useful method to detect the presence of transglutaminase in surimi-based products, which is derived from blood of different species of mammalian animals. Further study should be done to optimize the specificity of antibody used in the confirmation of TGase in surimi. © IDOSI Publications, 2012.
format Article
author A.R., Alina
M.A., Nur Illiyin
Y., Salmah
A., Siti Mashitoh
A.K., Imtinan
J., Juriani
author_facet A.R., Alina
M.A., Nur Illiyin
Y., Salmah
A., Siti Mashitoh
A.K., Imtinan
J., Juriani
author_sort A.R., Alina
title Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method
title_short Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method
title_full Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method
title_fullStr Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method
title_full_unstemmed Detection of Non-Halal Plasma Transglutaminase in Selected Surimi-Based Products by using Sandwich ELISA Method
title_sort detection of non-halal plasma transglutaminase in selected surimi-based products by using sandwich elisa method
publishDate 2015
url http://ddms.usim.edu.my/handle/123456789/8774
_version_ 1645152470441132032
score 13.211869

Similar Items